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Gontia, I.* ; Kavita, K.* ; Schmid, M. ; Hartmann, A. ; Jha, B.*

Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth promoting potential.

Int. J. Syst. Evol. Microbiol. 61, 2799-2804 (2011)
Verlagsversion DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A Gram-positive, aerobic, non-motile, coccoid shaped, halotolerant bacterium (JG 06 T) was isolated from roots of Salicornia brachiata, an extreme halophyte. Phylogenetic analysis based on 16S rRNA gene sequence showed sequence similarities of 99.2% to Brachybacterium paraconglomeratum, 99.0% to B. conglomeratum, and 98.2% to B. faecium. DNA-DNA hybridization with the close relatives Brachybacterium paraconglomeratum (DSM 46341T), B. conglomeratum (DSM 10241 T), B. faecium (DSM 4810T), B. tyrofermentans (DSM 10673 T), B. alimentarium (DSM 10672 T), B. fresconsis (DSM 14564 T), B. sacelli (DSM 14566 T) and B. muris (DSM 15460 T) resulted in re-association values of 36.2%, 36.5%, 35.8%, 27.6%, 27.9%, 28.2%, 28.7% and 11.2%, respectively. The peptidoglycan type of strain JG 06 T was variant A4γ. The menaquinone content was MK7 (100%). The polar lipid profile consisted of diphosphatidyglycerol, phosphatidyglycerol, monogalactosyl diglyceride and three unidentified phospholipids and three glycolipids. The predominant fatty acid was anteiso-C15:0 (52.07%); significant amounts of iso-C16:0(12.38%), iso-C15:0(8.59%) and anteiso-C17:0(10.03%) were also present. The G+C content of DNA was 73.0 mol%. The strain formed a growth pellicle in nitrogen-free semisolid NFb medium containing NaCl up to 4% and reduced acetylene to ethylene, characteristic for N2 fixation. In nutrient broth medium it grew up to 15% NaCl. It also had the ability to produce IAA, siderophore, utilized ACC as sole source of nitrogen and possessed ACC deaminase enzyme. On the basis of physiological, biochemical data and phylogeny, strain JG 06 T should be placed in the genus Brachybacterium. This bacterium represents a novel species of genus Brachybacterium for which the name Brachybacterium saurashtrense sp. nov. is proposed, with type strain JG 06 T (= DSM 23186T = IMCC 252T).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter no Keywords
ISSN (print) / ISBN 1466-5026
e-ISSN 1466-5034
Zeitschrift International Journal of Systematic and Evolutionary Microbiology
Quellenangaben Band: 61, Heft: 12, Seiten: 2799-2804 Artikelnummer: , Supplement: ,
Verlag SGM
Verlagsort Reading
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Microbe-Plant Interactions (AMP)