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Harris, C.* ; Scheibe, M.* ; Wongpalee, S.P.* ; Liu, W.* ; Cornett, E.M.* ; Vaughan, R.* ; Li, X.* ; Chen, W.* ; Xue, Y.* ; Zhong, Z.* ; Yen, L.* ; Barshop, W.D.* ; Rayatpisheh, S.* ; Gallego-Bartolome, J.* ; Groth, M. ; Wang, Z.* ; Wohlschlegel, J.A.* ; Du, J.* ; Rothbart, S.B.* ; Butter, F.* ; Jacobsen, S.E.*

A DNA methylation reader complex that enhances gene transcription.

Science 362, 1182-1186 (2018)
Postprint DOI PMC
Open Access Green
DNA methylation generally functions as a repressive transcriptional signal, but it is also known to activate gene expression. In either case, the downstream factors remain largely unknown. By using comparative interactomics, we isolated proteins in Arabidopsis thaliana that associate with methylated DNA. Two SU(VAR)3-9 homologs, the transcriptional antisilencing factor SUVH1, and SUVH3, were among the methyl reader candidates. SUVH1 and SUVH3 bound methylated DNA in vitro, were associated with euchromatic methylation in vivo, and formed a complex with two DNAJ domain-containing homologs, DNAJ1 and DNAJ2. Ectopic recruitment of DNAJ1 enhanced gene transcription in plants, yeast, and mammals. Thus, the SUVH proteins bind to methylated DNA and recruit the DNAJ proteins to enhance proximal gene expression, thereby counteracting the repressive effects of transposon insertion near genes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Zeitschrift Science
Quellenangaben Band: 362, Heft: 6419, Seiten: 1182-1186 Artikelnummer: , Supplement: ,
Verlag American Association for the Advancement of Science (AAAS)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)
Förderungen Howard Hughes Medical Institute
NIGMS NIH HHS