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Marlais, T.* ; Bickford-Smith, J.* ; Talavera Lopez, C.N. ; Le, H.* ; Chowdhury, F.* ; Miles, M.A.*

A comparative 'omics' approach for prediction of candidate Strongyloides stercoralis diagnostic coproantigens.

Plos Neglect. Trop. Dis. 17:e0010777 (2023)
Postprint DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Human infection with the intestinal nematode Strongyloides stercoralis is persistent unless effectively treated, and potentially fatal in immunosuppressed individuals. Epidemiological data are lacking, partially due to inadequate diagnosis. A rapid antigen detection test is a priority for population surveillance, validating cure after treatment, and for screening prior to immunosuppression. We used a targeted analysis of open access 'omics' data sets and used online predictors to identify S. stercoralis proteins that are predicted to be present in infected stool, Strongyloides-specific, and antigenic. Transcriptomic data from gut and non-gut dwelling life cycle stages of S. stercoralis revealed 328 proteins that are differentially expressed. Strongyloides ratti proteomic data for excreted and secreted (E/S) proteins were matched to S. stercoralis, giving 1,057 orthologues. Five parasitism-associated protein families (SCP/TAPS, prolyl oligopeptidase, transthyretin-like, aspartic peptidase, acetylcholinesterase) were compared phylogenetically between S. stercoralis and outgroups, and proteins with least homology to the outgroups were selected. Proteins that overlapped between the transcriptomic and proteomic datasets were analysed by multiple sequence alignment, epitope prediction and 3D structure modelling to reveal S. stercoralis candidate peptide/protein coproantigens. We describe 22 candidates from seven genes, across all five protein families for further investigation as potential S. stercoralis diagnostic coproantigens, identified using open access data and freely-available protein analysis tools. This powerful approach can be applied to many parasitic infections with 'omic' data to accelerate development of specific diagnostic assays for laboratory or point-of-care field application.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Ancylostoma-caninum; Protective Immunity; Infection; Parasite; Assays; Identification; Venezuelensis; Immunization; Sensitivity; Peptides
Sprache englisch
Veröffentlichungsjahr 2023
HGF-Berichtsjahr 2023
ISSN (print) / ISBN 1935-2727
e-ISSN 1935-2735
Zeitschrift PLoS Neglected Tropical Diseases
Quellenangaben Band: 17, Heft: 4, Seiten: , Artikelnummer: e0010777 Supplement: ,
Verlag Public Library of Science (PLoS)
Verlagsort 1160 Battery Street, Ste 100, San Francisco, Ca 94111 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Computational Biology (ICB)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503800-001
Förderungen John Henry Memorial Fund (UK Charity)
Sir Halley Stewart Trust
DOI 10.1371/journal.pntd.0010777
WOS ID 000975022400001
Scopus ID 85154610798
PubMed ID 37068106
Erfassungsdatum 2023-10-06
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