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Kozak, E.L. ; Miranda-Rodríguez, J.R. ; Borges, A. ; Dierkes, K.* ; Mineo, A.* ; Pinto-Teixeira, F.* ; Viader Llargues, O. ; Solon, J.* ; Chara, O.* ; López-Schier, H.

Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo.

Development 150:14 (2023)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Collective cell rotations are widely used during animal organogenesis. Theoretical and in vitro studies have conceptualized rotating cells as identical rigid-point objects that stochastically break symmetry to move monotonously and perpetually within an inert environment. However, it is unclear whether this notion can be extrapolated to a natural context, where rotations are ephemeral and heterogeneous cellular cohorts interact with an active epithelium. In zebrafish neuromasts, nascent sibling hair cells invert positions by rotating ≤180° around their geometric center after acquiring different identities via Notch1a-mediated asymmetric repression of Emx2. Here, we show that this multicellular rotation is a three-phasic movement that progresses via coherent homotypic coupling and heterotypic junction remodeling. We found no correlation between rotations and epithelium-wide cellular flow or anisotropic resistive forces. Moreover, the Notch/Emx2 status of the cell dyad does not determine asymmetric interactions with the surrounding epithelium. Aided by computer modeling, we suggest that initial stochastic inhomogeneities generate a metastable state that poises cells to move and spontaneous intercellular coordination of the resulting instabilities enables persistently directional rotations, whereas Notch1a-determined symmetry breaking buffers rotational noise.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Multicellular Rotations ; Patterning ; Regeneration ; Symmetry Breaking ; Zebrafish; Gene-expression; Motion; Regeneration; Migration; Drosophila; Establishment; Morphogenesis; Segmentation; Organization; Mechanics
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Zeitschrift Development / Company of Biologists
Quellenangaben Band: 150, Heft: 9, Seiten: , Artikelnummer: 14 Supplement: ,
Verlag Company of Biologists
Verlagsort Bidder Building, Station Rd, Histon, Cambridge Cb24 9lf, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Sensory Biology and Organogenesis (SBO)
Förderungen Marie Curie Actions (MSCA)
Helmholtz-Gemeinschaft
New York University Abu Dhabi
School of Biosciences, University of Nottingham
Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
Fondo para la Investigacion Cientifica y Tecnologica
European Union