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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Endogenous galectin-1 modulates cell biological properties of immortalized retinal pigment epithelial cells in vitro.
Int. J. Mol. Sci. 24:18 (2023)
In the eye, an increase in galectin-1 is associated with various chorioretinal diseases, in which retinal pigment epithelium (RPE) cells play a crucial role in disease development and progression. Since little is known about the function of endogenous galectin-1 in these cells, we developed a galectin-1-deficient immortalized RPE cell line (ARPE-19-LGALS1-/-) using a sgRNA/Cas9 all-in-one expression vector and investigated its cell biological properties. Galectin-1 deficiency was confirmed by Western blot analysis and immunocytochemistry. Cell viability and proliferation were significantly decreased in ARPE-19-LGALS1-/- cells when compared to wild-type controls. Further on, an increased attachment of galectin-1-deficient RPE cells was observed by cell adhesion assay when compared to control cells. The diminished viability and proliferation, as well as the enhanced adhesion of galectin-1-deficient ARPE-19 cells, could be blocked, at least in part, by the additional treatment with human recombinant galectin-1. In addition, a significantly reduced migration was detected in ARPE-19-LGALS1-/- cells. In comparison to control cells, galectin-1-deficient RPE cells had enhanced expression of sm-α-actin and N-cadherin, whereas expression of E-cadherin showed no significant alteration. Finally, a compensatory expression of galectin-8 mRNA was observed in ARPE-19-LGALS1-/- cells. In conclusion, in RPE cells, endogenous galectin-1 has crucial functions for various cell biological processes, including viability, proliferation, migration, adherence, and retaining the epithelial phenotype.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Arpe-19 ; Cell Attachment ; Cell Proliferation ; Cell Viability ; Compensatory Gene Expression ; Epithelial–mesenchymal Transition ; Galectin-1 ; Galectin-1 Deficiency ; Galectin-1 Knockout ; Retinal Pigment Epithelium Cells; Mesenchymal Transition; Migration; Proliferation; Inhibition; Antibodies; Attachment; Subunit; Protein
ISSN (print) / ISBN
1422-0067
e-ISSN
1661-6596
Zeitschrift
International Journal of Molecular Sciences
Quellenangaben
Band: 24,
Heft: 16,
Artikelnummer: 18
Verlag
MDPI
Verlagsort
Basel
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Structural Biology (STB)
Förderungen
The authors would like to thank Katja Widholzer for her excellent technical assistance.