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Pena-Francesch, M.* ; Vanoaica, L.D.* ; Zhu, G.F.* ; Stumpe, M.* ; Sankar, D.S.* ; Nowag, H.* ; Valencia-Camargo, A.D.* ; Hammerschmidt, W. ; Dengjel, J.* ; Ligeon, L.A.* ; Münz, C.*

The autophagy machinery interacts with EBV capsids during viral envelope release.

Proc. Natl. Acad. Sci. U.S.A. 120:e2211281120 (2023)
DOI PMC
Autophagy serves as a defense mechanism against intracellular pathogens, but several microorganisms exploit it for their own benefit. Accordingly, certain herpesviruses include autophagic membranes into their infectious virus particles. In this study, we analyzed the composition of purified virions of the Epstein-Barr virus (EBV), a common oncogenic γ-herpesvirus. In these, we found several components of the autophagy machinery, including membrane-associated LC3B-II, and numerous viral proteins, such as the capsid assembly proteins BVRF2 and BdRF1. Additionally, we showed that BVRF2 and BdRF1 interact with LC3B-II via their common protein domain. Using an EBV mutant, we identified BVRF2 as essential to assemble mature capsids and produce infectious EBV. However, BdRF1 was sufficient for the release of noninfectious viral envelopes as long as autophagy was not compromised. These data suggest that BVRF2 and BdRF1 are not only important for capsid assembly but together with the LC3B conjugation complex of ATG5-ATG12-ATG15L1 are also critical for EBV envelope release.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Ebv ; Autophagy ; Viral Capsid Assembly ; Viral Envelope ; Xenophagy; Epstein-barr-virus; Extracellular Vesicles; Proteins; Replication; Infection; Blocks; Fusion; Roles; Motif; Flux
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Zeitschrift Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Band: 120, Heft: 34, Seiten: , Artikelnummer: e2211281120 Supplement: ,
Verlag National Academy of Sciences
Verlagsort 2101 Constitution Ave Nw, Washington, Dc 20418 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Gene Vector (AGV)
Förderungen Novartis
Cancer Research Center Zurich
Swiss NSF
Vontobel Foundation
Roche
Swiss MS Society
Swiss Vaccine Research Institute
Sobek Foundation
HMZ ImmunoTargET of the University of Zurich
Cancer Research Switzerland
Forschungskredit of the University of Zurich