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Rosowski, S. ; Remmert, C. ; Marder, M. ; Akishiba, M. ; Bushe, J. ; Feuchtinger, A. ; Platen, A. ; Ussar, S. ; Theis, F.J. ; Wiedenmann, S. ; Meier, M.

Single-cell characterization of neovascularization using hiPSC-derived endothelial cells in a 3D microenvironment.

Stem Cell Rep. 18, 1972-1986 (2023)
DOI PMC
Creative Commons Lizenzvertrag
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
The formation of vascular structures is fundamental for in vitro tissue engineering. Vascularization can enable the nutrient supply within larger structures and increase transplantation efficiency. We differentiated human induced pluripotent stem cells toward endothelial cells in 3D suspension culture. To investigate in vitro neovascularization and various 3D microenvironmental approaches, we designed a comprehensive single-cell transcriptomic study. Time-resolved single-cell transcriptomics of the endothelial and co-evolving mural cells gave insights into cell type development, stability, and plasticity. Transfer to a 3D hydrogel microenvironment induced neovascularization and facilitated tracing of migrating, coalescing, and tubulogenic endothelial cell states. During maturation, we monitored two pericyte subtypes evolving mural cells. Profiling cell-cell interactions between pericytes and endothelial cells revealed angiogenic signals during tubulogenesis. In silico discovered ligands were tested for their capability to attract endothelial cells. Our data, analyses, and results provide an in vitro roadmap to guide vascularization in future tissue engineering.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Culturing Technologies ; Inferred Ec-pericyte Interactions ; Microfluidic Ligand Assay ; Neovascularization ; Single-cell Mrna Sequencing ; Stem Cell-derived Endothelial Cells; Growth-factor; Vegf; Contributes; Generation; Derivation; Regulator; Migration; Promotes; Cxcr4
ISSN (print) / ISBN 2213-6711
Zeitschrift Stem Cell Reports
Quellenangaben Band: 18, Heft: 10, Seiten: 1972-1986 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Maryland Heights, MO
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
Research Unit Analytical Pathology (AAP)
Institute of Diabetes and Obesity (IDO)
Institute of Computational Biology (ICB)
Förderungen
BMBF - SprintD
Helmholtz Pioneer Campus
ERC