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Luo, S. ; Ru, J. ; Mirzaei, M.K. ; Xue, J. ; Peng, X. ; Ralser, A.* ; Luque, R.M.* ; Gerhard, M.* ; Deng, L.

Gut virome profiling identifies an association between temperate phages and colorectal cancer promoted by Helicobacter pylori infection.

Gut Microbes 15:2257291 (2023)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers worldwide. While a close correlation between chronic Helicobacter pylori infection and CRC has been reported, the role of the virome has been overlooked. Here, we infected Apc-mutant mouse models and C57BL/6 mice with H. pylori and conducted a comprehensive metagenomics analysis of H. pylori-induced changes in lower gastrointestinal tract bacterial and viral communities. We observed an expansion of temperate phages in H. pylori infected Apc +/1638N mice at the early stage of carcinogenesis. Some of the temperate phages were predicted to infect bacteria associated with CRC, including Enterococcus faecalis. We also observed a high prevalence of virulent genes, such as flgJ, cwlJ, and sleB, encoded by temperate phages. In addition, we identified phages associated with pre-onset and onset of H. pylori-promoted carcinogenesis. Through co-occurrence network analysis, we found strong associations between the viral and bacterial communities in infected mice before the onset of carcinogenesis. These findings suggest that the expansion of temperate phages, possibly caused by prophage induction triggered by H. pylori infection, may have contributed to the development of CRC in mice by interacting with the bacterial community.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Auxiliary Metabolic Genes ; Bacteria-phage Interaction ; Colorectal Cancer ; Helicobacter Pylori ; Temperate Bacteriophage; Trimeric Autotransporter; Gastric-cancer; Inflammation; Populations; Adhesin; Gene; Caga
ISSN (print) / ISBN 1949-0976
e-ISSN 1949-0984
Zeitschrift Gut Microbes
Quellenangaben Band: 15, Heft: 2, Seiten: , Artikelnummer: 2257291 Supplement: ,
Verlag Landes Bioscience
Verlagsort 530 Walnut Street, Ste 850, Philadelphia, Pa 19106 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)
Förderungen The authors thank Sophie E. Smith for proofreading the manuscript, the reviewers and the editor for their constructive comments, as well as members of the Deng Lab for technical support.