PuSH - Publikationsserver des Helmholtz Zentrums München

Han, Y. ; Haouel, A. ; Georgii, E. ; Priego-Cubero, S.* ; Wurm, C. ; Hemmler, D. ; Schmitt-Kopplin, P. ; Becker, C.* ; Durner, J. ; Lindermayr, C.

Histone deacetylases HD2A and HD2B undergo feedback regulation by ABA and modulate drought tolerance via mediating ABA-induced transcriptional repression.

Genes 14:27 (2023)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Histone deacetylation catalyzed by histone deacetylase plays a critical role in gene silencing and subsequently controls many important biological processes. It was reported that the expression of the plant-specific histone deacetylase subfamily HD2s is repressed by ABA in Arabidopsis. However, little is known about the molecular relationship between HD2A/HD2B and ABA during the vegetative phase. Here, we describe that the hd2ahd2b mutant shows hypersensitivity to exogenous ABA during the germination and post-germination period. Additionally, transcriptome analyses revealed that the transcription of ABA-responsive genes was reprogrammed and the global H4K5ac level is specifically up-regulated in hd2ahd2b plants. ChIP-Seq and ChIP-qPCR results further verified that both HD2A and HD2B could directly and specifically bind to certain ABA-responsive genes. As a consequence, Arabidopsis hd2ahd2b plants displayed enhanced drought resistance in comparison to WT, which is consistent with increased ROS content, reduced stomatal aperture, and up-regulated drought-resistance-related genes. Moreover, HD2A and HD2B repressed ABA biosynthesis via the deacetylation of H4K5ac at NCED9. Taken together, our results indicate that HD2A and HD2B partly function through ABA signaling and act as negative regulators during the drought resistance response via the regulation of ABA biosynthesis and response genes.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Aba ; Arabidopsis Thaliana ; Drought Resistance ; Histone Acetylation ; Plant-specific Histone Deacetylases; Abscisic-acid Responses; 9-cis-epoxycarotenoid Dioxygenase; Expression Analysis; Stress Tolerance; Plant-responses; Gene-expression; Arabidopsis; Protein; Hda6; Channels
ISSN (print) / ISBN 2073-4425
e-ISSN 2073-4425
Zeitschrift Genes
Quellenangaben Band: 14, Heft: 6, Seiten: , Artikelnummer: 27 Supplement: ,
Verlag MDPI
Verlagsort St Alban-anlage 66, Ch-4052 Basel, Switzerland
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)
Research Unit Analytical BioGeoChemistry (BGC)
Institute of Lung Biology (LHI)