Startseite
English
Erweiterte Suche
Durchblättern nach ...
Publikationen im Überblick
Ansprechpartner
Hilfe
DOI
 |
|
|
möglich sobald bei der ZB eingereicht worden ist.
A photoreceptor-based hydrogel with red light-responsive reversible sol-gel transition as transient cellular matrix.
Adv. Mater. Technol. 8, 10:2300195 (2023)
Hydrogels with adjustable mechanical properties have been engineered as matrices for mammalian cells and allow the dynamic, mechano-responsive manipulation of cell fate and function. Recent research yields hydrogels, where biological photoreceptors translated optical signals into a reversible and adjustable change in hydrogel mechanics. While their initial application provides important insights into mechanobiology, broader implementation is limited by a small dynamic range of addressable stiffness. Herein, this limitation is overcome by developing a photoreceptor-based hydrogel with reversibly adjustable stiffness from ≈800 Pa to the sol state. The hydrogel is based on star-shaped polyethylene glycol, functionalized with the red/far-red light photoreceptor phytochrome B (PhyB), or phytochrome-interacting factor 6 (PIF6). Upon illumination with red light, PhyB heterodimerizes with PIF6, thus crosslinking the polymers and resulting in gelation. However, upon illumination with far-red light, the proteins dissociate and trigger a complete gel-to-sol transition. The hydrogel's light-responsive mechanical properties are comprehensively characterized and it is applied as a reversible extracellular matrix for the spatiotemporally controlled deposition of mammalian cells within a microfluidic chip. It is anticipated that this technology will open new avenues for the site- and time-specific positioning of cells and will contribute to overcome spatial restrictions.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Cell Deposition ; Cellular Matrix ; Hydrogels ; Materials ; Microfluidics ; Optogenetics; In-vivo; Wavelength; Culture; Growth
ISSN (print) / ISBN
2365-709X
e-ISSN
2365-709X
Zeitschrift
Advanced Materials Technologies
Quellenangaben
Band: 8,
Heft: 16,
Seiten: 10,
Artikelnummer: 2300195
Verlag
Wiley
Verlagsort
111 River St, Hoboken, Nj 07030 Usa
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Helmholtz Pioneer Campus (HPC)
Förderungen
European Research Council (ERC)
Projekt DEAL
Ministry for Science, Research and Arts of the state of Baden-Wurttemberg
Excellence Initiative of the German Federal and State Governments - BIOSS
German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) under Germany's Excellence Strategy - CIBSS
European Union (ERC)
Projekt DEAL
Ministry for Science, Research and Arts of the state of Baden-Wurttemberg
Excellence Initiative of the German Federal and State Governments - BIOSS
German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) under Germany's Excellence Strategy - CIBSS
European Union (ERC)