Startseite
English
Erweiterte Suche
Durchblättern nach ...
Publikationen im Überblick
Ansprechpartner
Hilfe
Verlagsversion
DOI
PMC
 |
Open Access Gold |
|
möglich sobald bei der ZB eingereicht worden ist.
Ascorbate deficiency increases progression of shigellosis in guinea pigs and mice infection models.
Gut Microbes 15:2271597 (2023)
Shigella spp. are the causative agents of bacterial dysentery and shigellosis, mainly in children living in developing countries. The study of Shigella entire life cycle in vivo and the evaluation of vaccine candidates' protective efficacy have been hampered by the lack of a suitable animal model of infection. None of the studies evaluated so far (rabbit, guinea pig, mouse) allowed the recapitulation of full shigellosis symptoms upon Shigella oral challenge. Historical reports have suggested that dysentery and scurvy are both metabolic diseases associated with ascorbate deficiency. Mammals, which are susceptible to Shigella infection (humans, non-human primates and guinea pigs) are among the few species unable to synthesize ascorbate. We optimized a low-ascorbate diet to induce moderate ascorbate deficiency, but not scurvy, in guinea pigs to investigate whether poor vitamin C status increases the progression of shigellosis. Moderate ascorbate deficiency increased shigellosis symptom severity during an extended period of time (up to 48 h) in all strains tested (Shigella sonnei, Shigella flexneri 5a, and 2a). At late time points, an important influx of neutrophils was observed both within the disrupted colonic mucosa and in the luminal compartment, although Shigella was able to disseminate deep into the organ to reach the sub-mucosal layer and the bloodstream. Moreover, we found that ascorbate deficiency also increased Shigella penetration into the colon epithelium layer in a Gulo-/- mouse infection model. The use of these new rodent models of shigellosis opens new doors for the study of both Shigella infection strategies and immune responses to Shigella infection.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Animal Models Of Gi Infection Or Gi-diseases With Microbial Components. ; Shigella ; Ascorbate Deficiency ; Infection Animal Model ; Intestinal Infection ; Shigellosis; Vitamin-c; Children; Supplementation; Dysentery; Apoptosis; Diarrhea
ISSN (print) / ISBN
1949-0976
e-ISSN
1949-0984
Zeitschrift
Gut Microbes
Quellenangaben
Band: 15,
Heft: 2,
Artikelnummer: 2271597
Verlag
Landes Bioscience
Verlagsort
530 Walnut Street, Ste 850, Philadelphia, Pa 19106 Usa
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Analytical Pathology (AAP)
Förderungen
Institut Pasteur Histology Platform for processing the colonic tissues of guinea pigs
We thank Patricia Flamant from the Institut Pasteur Histology Platform for processing the colonic tissues of guinea pigs. We are very grateful to the team of the Institut Pasteur Animalerie Centrale for their constant support with this project (particular
We thank Patricia Flamant from the Institut Pasteur Histology Platform for processing the colonic tissues of guinea pigs. We are very grateful to the team of the Institut Pasteur Animalerie Centrale for their constant support with this project (particular