PuSH - Publikationsserver des Helmholtz Zentrums München: Immunological quantitation of the glycation site lysine-414 in serum albumin in human plasma samples by indirect ELISA using highly specific monoclonal antibodies.

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Mötzing, M.* ; Blüher, M. ; Grunwald, T.* ; Hoffmann, R.*

Immunological quantitation of the glycation site lysine-414 in serum albumin in human plasma samples by indirect ELISA using highly specific monoclonal antibodies.

ChemBioChem:e202300550 (2023)

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Diabetes mellitus, a metabolic disorder that is characterized by elevated blood glucose levels, is common throughout the world and its prevalence is steadily increasing. Early diagnosis and treatment are important to prevent acute complications and life-threatening long-term organ damage. Glycation sites in human serum albumin (HSA) are considered to be promising biomarkers of systemic glycemic status. This work aimed to develop a sensitive and clinically applicable ELISA for the quantification of glycation site Lys414 in HSA (HSA_K414 ). The monoclonal antibodies (mAbs) were generated by immunizing mice with a glycated peptide. The established indirect ELISA based on mAb 50D8 (IgG₁ isotype) yielded a limit of detection of 0.39 nmol/g HSA for HSA_K414 with a linear dynamic range from 0.50 to 6.25 nmol/g glycated HSA. The inter- and intra-day assays with coefficients of variation less than 20 % indicated good assay performance and precision. Assay evaluation was based on plasma samples from diabetic and non-diabetic subjects with known HSA_K414 glycation levels previously determined by LC-MS. Both data sets correlated very well. In conclusion, the generated mAb 50D8 and the established ELISA could be a valuable tool for the rapid quantitation of glycation site HSA_K414 in plasma samples to evaluate its clinical relevance.

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