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Mötzing, M.* ; Blüher, M. ; Grunwald, T.* ; Hoffmann, R.*

Immunological quantitation of the glycation site lysine-414 in serum albumin in human plasma samples by indirect ELISA using highly specific monoclonal antibodies.

ChemBioChem:e202300550 (2023)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Diabetes mellitus, a metabolic disorder that is characterized by elevated blood glucose levels, is common throughout the world and its prevalence is steadily increasing. Early diagnosis and treatment are important to prevent acute complications and life-threatening long-term organ damage. Glycation sites in human serum albumin (HSA) are considered to be promising biomarkers of systemic glycemic status. This work aimed to develop a sensitive and clinically applicable ELISA for the quantification of glycation site Lys414 in HSA (HSAK414 ). The monoclonal antibodies (mAbs) were generated by immunizing mice with a glycated peptide. The established indirect ELISA based on mAb 50D8 (IgG1 isotype) yielded a limit of detection of 0.39 nmol/g HSA for HSAK414 with a linear dynamic range from 0.50 to 6.25 nmol/g glycated HSA. The inter- and intra-day assays with coefficients of variation less than 20 % indicated good assay performance and precision. Assay evaluation was based on plasma samples from diabetic and non-diabetic subjects with known HSAK414 glycation levels previously determined by LC-MS. Both data sets correlated very well. In conclusion, the generated mAb 50D8 and the established ELISA could be a valuable tool for the rapid quantitation of glycation site HSAK414 in plasma samples to evaluate its clinical relevance.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Diabetes Mellitus ; Human Serum Albumin (hsa) ; Immunoassays ; Monoclonal Antibodies (mabs) ; Protein Glycation
ISSN (print) / ISBN 1439-4227
e-ISSN 1439-7633
Zeitschrift ChemBioChem
Quellenangaben Band: , Heft: , Seiten: , Artikelnummer: e202300550 Supplement: ,
Verlag Wiley
Verlagsort Postfach 101161, 69451 Weinheim, Germany
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Förderungen European Social Fund (ESF)