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Arias, A. ; Anastasopoulou, M. ; Gorpas, D. ; Ntziachristos, V.

Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering.

Biomed. Opt. Express 14, 5499-5511 (2023)
Verlagsversion DOI PMC
Open Access Gold
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The total diffuse reflectance RT and the effective attenuation coefficient µeff of an optically diffuse medium map uniquely onto its absorption and reduced scattering coefficients. Using this premise, we developed a methodology where RT and the slope of the logarithmic spatially resolved reflectance, a quantity related to µeff, are the inputs of a look-up table to correct the dependence of fluorescent signals on the media's optical properties. This methodology does not require an estimation of the medium's optical property, avoiding elaborate simulations and their errors to offer accurate and fast corrections. The experimental demonstration of our method yielded a mean relative error in fluorophore concentrations of less than 4% over a wide range of optical property variations. We discuss how the method developed can be employed to improve image fidelity and fluorochrome quantification in fluorescence molecular imaging clinical applications.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter In-vivo Quantification; Real-time; Surface-roughness; Molecular Markers; Turbid Media; Spectroscopy; Reflectance; Light; Tissues; System
Sprache englisch
Veröffentlichungsjahr 2023
HGF-Berichtsjahr 2023
ISSN (print) / ISBN 2156-7085
e-ISSN 2156-7085
Zeitschrift Biomedical Optics Express
Quellenangaben Band: 14, Heft: 10, Seiten: 5499-5511 Artikelnummer: , Supplement: ,
Verlag Optical Society of America (OSA)
Verlagsort 2010 Massachusetts Ave Nw, Washington, Dc 20036 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505500-001
Förderungen Horizon Europe - Pillar III
European Union Horizon Europe
Deutsches Zentrum fuer Herz-Kreislaufforschung
Gottfried Wilhelm Leibniz
Deutsche Forschungsgemeinschaft
DOI 10.1364/BOE.496599
WOS ID 001088385200003
Scopus ID 85174339561
PubMed ID 37854563
Erfassungsdatum 2023-11-28
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