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Arias, A. ; Anastasopoulou, M. ; Gorpas, D. ; Ntziachristos, V.

Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering.

Biomed. Opt. Express 14, 5499-5511 (2023)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The total diffuse reflectance RT and the effective attenuation coefficient µeff of an optically diffuse medium map uniquely onto its absorption and reduced scattering coefficients. Using this premise, we developed a methodology where RT and the slope of the logarithmic spatially resolved reflectance, a quantity related to µeff, are the inputs of a look-up table to correct the dependence of fluorescent signals on the media's optical properties. This methodology does not require an estimation of the medium's optical property, avoiding elaborate simulations and their errors to offer accurate and fast corrections. The experimental demonstration of our method yielded a mean relative error in fluorophore concentrations of less than 4% over a wide range of optical property variations. We discuss how the method developed can be employed to improve image fidelity and fluorochrome quantification in fluorescence molecular imaging clinical applications.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter In-vivo Quantification; Real-time; Surface-roughness; Molecular Markers; Turbid Media; Spectroscopy; Reflectance; Light; Tissues; System
ISSN (print) / ISBN 2156-7085
e-ISSN 2156-7085
Zeitschrift Biomedical Optics Express
Quellenangaben Band: 14, Heft: 10, Seiten: 5499-5511 Artikelnummer: , Supplement: ,
Verlag Optical Society of America (OSA)
Verlagsort 2010 Massachusetts Ave Nw, Washington, Dc 20036 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Förderungen Horizon Europe - Pillar III
European Union Horizon Europe
Deutsches Zentrum fuer Herz-Kreislaufforschung
Gottfried Wilhelm Leibniz
Deutsche Forschungsgemeinschaft