Startseite
English
Erweiterte Suche
Durchblättern nach ...
Publikationen im Überblick
Ansprechpartner
Hilfe
Verlagsversion
DOI
PMC
 |
Open Access Gold |
|
möglich sobald bei der ZB eingereicht worden ist.
NEUROD2 function is dispensable for human pancreatic β cell specification.
Front. Endocrin. 14:1286590 (2023)
INTRODUCTION: The molecular programs regulating human pancreatic endocrine cell induction and fate allocation are not well deciphered. Here, we investigated the spatiotemporal expression pattern and the function of the neurogenic differentiation factor 2 (NEUROD2) during human endocrinogenesis. METHODS: Using Crispr-Cas9 gene editing, we generated a reporter knock-in transcription factor (TF) knock-out human inducible pluripotent stem cell (iPSC) line in which the open reading frame of both NEUROD2 alleles are replaced by a nuclear histone 2B-Venus reporter (NEUROD2nVenus/nVenus). RESULTS: We identified a transient expression of NEUROD2 mRNA and its nuclear Venus reporter activity at the stage of human endocrine progenitor formation in an iPSC differentiation model. This expression profile is similar to what was previously reported in mice, uncovering an evolutionarily conserved gene expression pattern of NEUROD2 during endocrinogenesis. In vitro differentiation of the generated homozygous NEUROD2nVenus/nVenus iPSC line towards human endocrine lineages uncovered no significant impact upon the loss of NEUROD2 on endocrine cell induction. Moreover, analysis of endocrine cell specification revealed no striking changes in the generation of insulin-producing b cells and glucagon-secreting a cells upon lack of NEUROD2. DISCUSSION: Overall, our results suggest that NEUROD2 is expendable for human b cell formation in vitro.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Neurod2 ; Endocrine Cells ; Endocrinogenesis ; Ipsc Differentiation ; β Cells; Differentiation; Neurogenin3; System; Genes
ISSN (print) / ISBN
1664-2392
e-ISSN
1664-2392
Zeitschrift
Frontiers in Endocrinology
Quellenangaben
Band: 14,
Artikelnummer: 1286590
Verlag
Frontiers
Verlagsort
Lausanne
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Förderungen
DZD NEXT Young Talent Program
scholarship under the State Scholarship Fund by the China Scholarship Council
Helmholtz Research School for Diabetes (HRD) - Helmholtz Association - Initiative and Networking Fund (IVF)
German Center for Diabetes Research (DZD e.V.)
Helmholtz Portfolio Theme'Metabolic Dysfunction and Common Disease
Helmholtz Society
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The work was supported by the Helmholtz Society, Helmholtz Portfolio Theme'Metabolic Dysfunction and Common Disease, and German Center f
scholarship under the State Scholarship Fund by the China Scholarship Council
Helmholtz Research School for Diabetes (HRD) - Helmholtz Association - Initiative and Networking Fund (IVF)
German Center for Diabetes Research (DZD e.V.)
Helmholtz Portfolio Theme'Metabolic Dysfunction and Common Disease
Helmholtz Society
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The work was supported by the Helmholtz Society, Helmholtz Portfolio Theme'Metabolic Dysfunction and Common Disease, and German Center f