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Waszczak, C.* ; Yarmolinsky, D.* ; Leal Gavarrón, M.* ; Vahisalu, T.* ; Sierla, M.* ; Zamora, O.* ; Carter, R.* ; Puukko, T.* ; Sipari, N.* ; Lamminmäki, A.* ; Durner, J. ; Ernst, D. ; Winkler, J.B. ; Paulin, L.* ; Auvinen, P.* ; Fleming, A.J.* ; Andersson, M.X.* ; Kollist, H.* ; Kangasjärvi, J.*

Synthesis and import of GDP-l-fucose into the Golgi affect plant-water relations.

New Phytol. 241, 747-763 (2023)
Postprint DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Land plants evolved multiple adaptations to restrict transpiration. However, the underlying molecular mechanisms are not sufficiently understood. We used an ozone-sensitivity forward genetics approach to identify Arabidopsis thaliana mutants impaired in gas exchange regulation. High water loss from detached leaves and impaired decrease of leaf conductance in response to multiple stomata-closing stimuli were identified in a mutant of MURUS1 (MUR1), an enzyme required for GDP-l-fucose biosynthesis. High water loss observed in mur1 was independent from stomatal movements and instead could be linked to metabolic defects. Plants defective in import of GDP-l-Fuc into the Golgi apparatus phenocopied the high water loss of mur1 mutants, linking this phenotype to Golgi-localized fucosylation events. However, impaired fucosylation of xyloglucan, N-linked glycans, and arabinogalactan proteins did not explain the aberrant water loss of mur1 mutants. Partial reversion of mur1 water loss phenotype by borate supplementation and high water loss observed in boron uptake mutants link mur1 gas exchange phenotypes to pleiotropic consequences of l-fucose and boron deficiency, which in turn affect mechanical and morphological properties of stomatal complexes and whole-plant physiology. Our work emphasizes the impact of fucose metabolism and boron uptake on plant-water relations.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cell Wall ; Fucose Metabolism ; Plant-water Relations ; Rhamnogalacturonan Ii ; Stomata; De-novo Synthesis; Rhamnogalacturonan-ii; Plasma-membrane; Cross-linking; Cell-walls; Protein Fucosylation; Mur1 Gene; Arabidopsis; Mutant; Boron
ISSN (print) / ISBN 0028-646X
e-ISSN 1469-8137
Zeitschrift New Phytologist
Quellenangaben Band: 241, Heft: 2, Seiten: 747-763 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)
Research Unit Environmental Simulation (BIOP-EUS)
Förderungen Suomen Kulttuurirahasto
Opetushallitus
Helsingin Yliopisto
European Regional Development Fund
Ella ja Georg Ehrnroothin Säätiö