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Hils, M.* ; Hoffard, N.* ; Iuliano, C.* ; Kreft, L. ; Chakrapani, N.* ; Swiontek, K.* ; Fischer, K.* ; Eberlein, B.* ; Köberle, M.* ; Fischer, J.* ; Hilger, C.* ; Ohnmacht, C. ; Kaesler, S.* ; Wölbing, F.* ; Biedermann, T.*

IgE and anaphylaxis specific to the carbohydrate alpha-gal depend on interleukin-4.

J. Allergy Clin. Immunol. 153, 1050-1062.e6 (2023)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
BACKGROUND: Alpha-gal (Galα1-3Galβ1-4GlcNAc) is a carbohydrate with the potential to elicit fatal allergic reactions to mammalian meat and drugs of mammalian origin. This type of allergy is induced by tick bites and therapeutic options for this skin-driven food allergy are limited to the avoidance of the allergen and treatment of symptoms. Thus, a better understanding of the immune mechanisms resulting in sensitization through the skin is crucial, especially in the case of a carbohydrate allergen for which underlying immune responses are poorly understood. OBJECTIVE: We aimed to establish a mouse model of alpha-gal allergy for in-depth immunological analyses. METHODS: GGTA1-deficient mice devoid of alpha-gal glycosylations were sensitized with the alpha-gal-carrying self-protein mouse serum albumin via repetitive intracutaneous injections in combination with the adjuvant aluminum hydroxide. The role of basophils and IL-4 in sensitization was investigated by using antibody-mediated depletion. RESULTS: Alpha-gal-sensitized mice displayed increased levels of alpha-gal-specific IgE and IgG1 and developed systemic anaphylaxis upon challenge with both alpha-gal-containing glycoproteins and glycolipids. In accordance with alpha-gal-allergic patients, we detected elevated numbers of basophils at the site of sensitization as well as increased numbers of alpha-gal-specific B cells, germinal center B cells and B cells of IgE and IgG1 isotypes in skin-draining lymph nodes. By depleting IL-4 during sensitization, we demonstrated for the first time that sensitization and elicitation of allergy to alpha-gal and correspondingly to a carbohydrate allergen is dependent on IL-4. CONCLUSION: These findings establish IL-4 as a potential target to interfere with alpha-gal allergy elicited by tick bites.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Ggta1-deficient Mouse Model ; Galα1-3galβ1-4glcnac ; Il-4 ; Ige ; Alpha-gal Syndrome ; Anaphylaxis ; Food Allergy ; Red Meat Allergy; Tick Bites; Mammalian Meat; Allergy; Sensitization; Antibodies; Proteins; Glycans; Model
ISSN (print) / ISBN 0091-6749
e-ISSN 1097-6825
Zeitschrift The journal of allergy and clinical immunology
Quellenangaben Band: 153, Heft: 4, Seiten: 1050-1062.e6 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Förderungen European Research Council (ERC)
Luxembourg National Research Fund (FNR)
DFG