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Theobald, S.J.* ; Fiestas Carcaba, E. ; Schneider, A.* ; Ostermann, B.* ; Danisch, S.* ; von Kaisenberg, C.* ; Rybniker, J.* ; Hammerschmidt, W. ; Zeidler, R. ; Stripecke, R.*

Fully human herpesvirus-specific neutralizing IgG antibodies generated by EBV immortalization of splenocytes-derived from immunized humanized Mice.

Cells 13:13 (2024)
DOI PMC
Creative Commons Lizenzvertrag
Antiviral neutralizing antibodies (nAbs) are commonly derived from B cells developed in immunized or infected animals and humans. Fully human antibodies are preferred for clinical use as they are potentially less immunogenic. However, the function of B cells varies depending on their homing pattern and an additional hurdle for antibody discovery in humans is the source of human tissues with an immunological microenvironment. Here, we show an efficient method to pharm human antibodies using immortalized B cells recovered from Nod.Rag.Gamma (NRG) mice reconstituting the human immune system (HIS). Humanized HIS mice were immunized either with autologous engineered dendritic cells expressing the human cytomegalovirus gB envelope protein (HCMV-gB) or with Epstein–Barr virus-like particles (EB-VLP). Human B cells recovered from spleen of HIS mice were efficiently immortalized with EBV in vitro. We show that these immortalized B cells secreted human IgGs with neutralization capacities against prototypic HCMV-gB and EBV-gp350. Taken together, we show that HIS mice can be successfully used for the generation and pharming fully human IgGs. This technology can be further explored to generate antibodies against emerging infections for diagnostic or therapeutic purposes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Antibodies ; Dendritic Cells ; Ebv ; Hcmv ; Herpesvirus ; Humanized Mice ; Immortalization ; Virus-like Particles; Epstein-barr-virus; Mouse Model; Risk-factors; Cytomegalovirus; Infection; System; Impact; Blood
ISSN (print) / ISBN 2073-4409
e-ISSN 2073-4409
Zeitschrift Cells
Quellenangaben Band: 13 Heft: 1, Seiten: , Artikelnummer: 13 Supplement: ,
Verlag MDPI
Verlagsort Basel
Nichtpatentliteratur Publikationen
Institut(e) Institute of Structural Biology (STB)
Research Unit Gene Vector (AGV)
Förderungen Center for Molecular Medicine Cologne