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Mitic, N.* ; Neuschulz, A.* ; Spanjaard, B.* ; Schneider, J. ; Fresmann, N.* ; Novoselc, K.T. ; Strunk, T.* ; Münster, L.* ; Olivares-Chauvet, P.* ; Ninkovic, J. ; Junker, J.P.*

Dissecting the spatiotemporal diversity of adult neural stem cells.

Mol. Syst. Biol. 20, 321-337 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Adult stem cells are important for tissue turnover and regeneration. However, in most adult systems it remains elusive how stem cells assume different functional states and support spatially patterned tissue architecture. Here, we dissected the diversity of neural stem cells in the adult zebrafish brain, an organ that is characterized by pronounced zonation and high regenerative capacity. We combined single-cell transcriptomics of dissected brain regions with massively parallel lineage tracing and in vivo RNA metabolic labeling to analyze the regulation of neural stem cells in space and time. We detected a large diversity of neural stem cells, with some subtypes being restricted to a single brain region, while others were found globally across the brain. Global stem cell states are linked to neurogenic differentiation, with different states being involved in proliferative and non-proliferative differentiation. Our work reveals principles of adult stem cell organization and establishes a resource for the functional manipulation of neural stem cell subtypes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Massively Parallel Lineage Tracing ; Radial Glia ; Single-cell Rna Metabolic Labeling ; Single-cell Transcriptomics ; Zebrafish; Zebrafish Brain; Radial Glia; Neurogenesis; Proliferation; Migration; Origin; Injury; Model
ISSN (print) / ISBN 1744-4292
e-ISSN 1744-4292
Zeitschrift Molecular Systems Biology
Quellenangaben Band: 20, Heft: 4, Seiten: 321-337 Artikelnummer: , Supplement: ,
Verlag EMBO Press
Verlagsort Campus, 4 Crinan St, London, N1 9xw, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)
Förderungen Studienstiftung des deutschen Volkes
Helmholtz AI grant
Consolidator Grant
European Research Council Starting Grant
MDC/BIMSB core facilities (zebrafish, genomics, bioinformatics)