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Tsvilovskyy, V.* ; Ottenheijm, R.* ; Kriebs, U.* ; Schütz, A.* ; Diakopoulos, K.N.* ; Jha, A.N.* ; Bildl, W.* ; Wirth, A.* ; Böck, J.* ; Jaślan, D.* ; Ferro, I.* ; Taberner, F.J.* ; Kalinina, O.* ; Hildebrand, S.* ; Wissenbach, U.* ; Weissgerber, P.* ; Vogt, D.* ; Eberhagen, C. ; Mannebach, S.* ; Berlin, M.* ; Kuryshev, V.* ; Schumacher, D.* ; Philippaert, K.* ; Camacho-Londoño, J.E.* ; Mathar, I.* ; Dieterich, C.* ; Klugbauer, N.* ; Biel, M.* ; Wahl-Schott, C.* ; Lipp, P.* ; Flockerzi, V.* ; Zischka, H. ; Algül, H.* ; Lechner, S.G.* ; Lesina, M.* ; Grimm, C.* ; Fakler, B.* ; Schulte, U.* ; Muallem, S.* ; Freichel, M.*

OCaR1 endows exocytic vesicles with autoregulatory competence by preventing uncontrolled Ca2+ release, exocytosis, and pancreatic tissue damage.

J. Clin. Invest. 134:e169428 (2024)
Verlagsversion DOI PMC
Creative Commons Lizenzvertrag
Regulated exocytosis is initiated by increased Ca2+ concentrations in close spatial proximity to secretory granules, which is effectively prevented when the cell is at rest. Here we showed that exocytosis of zymogen granules in acinar cells was driven by Ca2+ directly released from acidic Ca2+ stores including secretory granules through NAADP-activated two-pore channels (TPCs). We identified OCaR1 (encoded by Tmem63a) as an organellar Ca2+ regulator protein integral to the membrane of secretory granules that controlled Ca2+ release via inhibition of TPC1 and TPC2 currents. Deletion of OCaR1 led to extensive Ca2+ release from NAADP-responsive granules under basal conditions as well as upon stimulation of GPCR receptors. Moreover, OCaR1 deletion exacerbated the disease phenotype in murine models of severe and chronic pancreatitis. Our findings showed OCaR1 as a gatekeeper of Ca2+ release that endows NAADP-sensitive secretory granules with an autoregulatory mechanism preventing uncontrolled exocytosis and pancreatic tissue damage.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Calcium Signaling ; Cell Biology ; Ion Channels ; Lysosomes; Cyclic Adp-ribose; Endoplasmic-reticulum; Inositol Trisphosphate; Zymogen Granules; Calcium; Naadp; Channels; Mechanism; Roles; Identification
ISSN (print) / ISBN 0021-9738
e-ISSN 1558-8238
Zeitschrift Journal of Clinical Investigation
Quellenangaben Band: 134, Heft: 7, Seiten: , Artikelnummer: e169428 Supplement: ,
Verlag American Society of Clinical Investigation
Verlagsort 2015 Manchester Rd, Ann Arbor, Mi 48104 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Toxicology and Pharmacology (TOXI)
Förderungen NIH
Research Training Group
Collaborative Research Centre (SFB)
DZHK (German Centre for Cardiovascular Research)
BMBF (German Ministry of Education and Research)
German Research Foundation (DFG)
Transregional Collaborative Research Centre (TR-SFB)