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Schiller, L. ; Ko, C.* ; Kosinska, A. ; Grimm, D.* ; Protzer, U.

Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication.

In: Hepatitis B Virus. Berlin [u.a.]: Springer, 2024. 207-218 (Methods Mol. Biol. ; 2837)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Mice infected with a recombinant adeno-associated virus carrying a replication-competent hepatitis B virus genome (rAAV-HBV) via the intravenous route establish a persistent HBV replication in hepatocytes and develop immune tolerance. They serve as models to evaluate antiviral immunity and to assess potential therapeutic approaches for chronic HBV infection. Combining selected HBV variants and different mouse genotypes allows for addressing a broad spectrum of research questions. This chapter describes the basic principles of the rAAV-HBV mouse model, rAAV-HBV production and purification methods, and finally, the in vivo application.
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Publikationstyp Artikel: Sammelbandbeitrag/Buchkapitel
Korrespondenzautor
Schlagwörter Adeno-associated Virus ; Cchronic Hbv Infection ; Gene Transfer ; Hbv Variants ; Hepatitis B Virus ; Iodixanol Purification ; Mouse Model
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Bandtitel Hepatitis B Virus
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 2837, Heft: , Seiten: 207-218 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Virology (VIRO)