PuSH - Publikationsserver des Helmholtz Zentrums München

Ayten, M.* ; Straub, T.* ; Kaplan, L.* ; Hauck, S.M. ; Grosche, A.* ; Koch, S.F.*

CD44 signaling in Müller cells impacts photoreceptor function and survival in healthy and diseased retinas.

J. Neuroinflamm. 21:190 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Retinitis pigmentosa (RP), an inherited retinal disease, affects 1,5 million people worldwide. The initial mutation-driven photoreceptor degeneration leads to chronic inflammation, characterized by Müller cell activation and upregulation of CD44. CD44 is a cell surface transmembrane glycoprotein and the primary receptor for hyaluronic acid. It is involved in many pathological processes, but little is known about CD44's retinal functions. CD44 expression is also increased in Müller cells from our Pde6bSTOP/STOP RP mouse model. To gain a more detailed understanding of CD44's role in healthy and diseased retinas, we analyzed Cd44-/- and Cd44-/-Pde6bSTOP/STOP mice, respectively. The loss of CD44 led to enhanced photoreceptor degeneration, reduced retinal function, and increased inflammatory response. To understand the underlying mechanism, we performed proteomic analysis on isolated Müller cells from Cd44-/- and Cd44-/-Pde6bSTOP/STOP retinas and identified a significant downregulation of glutamate transporter 1 (SLC1A2). This downregulation was accompanied by higher glutamate levels, suggesting impaired glutamate homeostasis. These novel findings indicate that CD44 stimulates glutamate uptake via SLC1A2 in Müller cells, which in turn, supports photoreceptor survival and function.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cd44 ; Gliosis ; Glutamate ; Inflammation ; Müller Cells ; Retinitis Pigmentosa ; Slc1a2; Muller Glia; Extracellular-matrix; Transcriptional Regulation; Glutamate Transporters; Retinitis-pigmentosa; Preclinical Model; Up-regulation; Inflammation; Hyaluronan; Expression
ISSN (print) / ISBN 1742-2094
e-ISSN 1742-2094
Zeitschrift Journal of Neuroinflammation
Quellenangaben Band: 21, Heft: 1, Seiten: , Artikelnummer: 190 Supplement: ,
Verlag BioMed Central
Verlagsort London
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Förderungen Ludwig-Maximilians-Universitt Mnchen (1024)