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Böckel, C. ; Pastor, X. ; Heinig, M. ; Walzthoeni, T.

Differential analysis of protein-DNA binding using ChIP-seq data.

In: Chromatin Immunoprecipitation. Berlin [u.a.]: Springer, 2024. 63-89 (Methods Mol. Biol. ; 2846)
DOI PMC
Chromatin immunoprecipitation in combination with next-generation sequencing (ChIP-Seq) allows probing of protein-DNA binding in a rapid and genome-wide fashion. Herein we describe the required steps to preprocess ChIP-Seq data and to analyze the differential binding of proteins to DNA for perturbation experiments. In these experiments, different conditions are compared to find the underlying biological mechanisms caused by the stimulus or treatment. In addition, we provide a sample analysis using the steps outlined in the chapter.
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Publikationstyp Artikel: Sammelbandbeitrag/Buchkapitel
Korrespondenzautor
Schlagwörter Diffbind ; Differential Chip-seq Analysis ; Chip-seq
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Bandtitel Chromatin Immunoprecipitation
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 2846, Heft: , Seiten: 63-89 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Genomics (CF-GEN)
Institute of Computational Biology (ICB)