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Chromatin immunoprecipitation in adipose tissue and adipocytes: How to proceed and optimize the protocol for transcription factor DNA binding.
In: Chromatin Immunoprecipitation. Berlin [u.a.]: Springer, 2024. 35-45 (Methods Mol. Biol. ; 2846)
Chromatin immunoprecipitation (ChIP) coupled to qPCR or sequencing is a crucial experiment to determine direct transcriptional regulation under the control of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels.Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipitation, and DNA purification. We also discuss critical steps for optimizing the experiment to perform a successful ChIP in lipid-rich cells/tissues.
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Publikationstyp
Artikel: Sammelbandbeitrag/Buchkapitel
Schlagwörter
Adipocyte ; Adipose Tissue ; Chip ; Chromatin Immunoprecipitation
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Bandtitel
Chromatin Immunoprecipitation
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 2846,
Seiten: 35-45
Verlag
Springer
Verlagsort
Berlin [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Diabetes and Endocrinology (IDE)
Institute of Diabetes and Cancer (IDC)
Institute of Diabetes and Cancer (IDC)