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Schneider, E. ; Amar, Y.* ; Butter, K.* ; Steiger, K.* ; Musiol, S. ; Garcia-Käufer, M.* ; Hölge, I.M.* ; Schnautz, B. ; Gschwendtner, S. ; Ghirardo, A. ; Gminski, R.* ; Eberlein, B.* ; Esser-von Bieren, J. ; Biedermann, T.* ; Haak, S. ; Ohlmeyer, M.* ; Schmidt-Weber, C.B. ; Eyerich, S. ; Alessandrini, F.

Pinewood VOC emissions protect from oxazolone-induced inflammation and dysbiosis in a mouse model of atopic dermatitis.

Environ. Int. 192:109035 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Pinewood, increasingly used in construction and interior fittings, emits high amounts of volatile organic compounds (VOCs), which tend to accumulate in indoor air. Whether indoor VOCs affect the development of atopic dermatitis (AD) is a matter of debate. We aimed to evaluate the effects of pinewood VOCs on the development of AD-like inflammatory phenotype and linked microbiome alterations, both hallmarks of AD. An oxazolone-induced mouse model of AD was exposed to three different VOC concentrations emitted by pinewood plates throughout the experiment. The disease course and associated immunological and microbiological changes were evaluated. To validate and translate our results to humans, human keratinocytes were exposed to a synthetic pinewood VOCs mixture in an AD environment. Pinewood emitted mainly terpenes, which at a total concentration of 5 mg/m3 significantly improved oxazolone-induced key AD parameters, such as serum total IgE, transepidermal water loss, barrier gene alteration, inflammation, and dysbiosis. Notably, exposure to pinewood VOCs restored the loss of microbial richness and inhibit Staphylococci expansion characteristic of the oxazolone-induced mouse AD model. Most beneficial effects of pinewood VOCs were dose-dependent. In fact, lower (<3 mg/m3) or higher (>10 mg/m3) pinewood VOC levels maintained only limited beneficial effects, such as preserving the microbiome richness or impeding Staphylococci expansion, respectively. In the human in-vitro model, exposure of keratinocytes grown in an AD environment to a pinewood VOCs mixture reduced the release of inflammatory markers. In conclusion, our results indicate that airborne phytochemicals emitted from pinewood have beneficial effects on an AD-like phenotype and associated dysbiosis. These investigations highlight the effects of terpenes as environmental compounds in the prevention and/or control of atopic skin disease.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Atopic Dermatitis ; Indoor Environment ; Microbiome ; Terpenes ; Voc Emissions; Volatile Organic-compounds; Alpha-pinene; Aureus Colonization; Essential Oil; Skin-lesions; Indoor Air; Exposure; Cytokines; Quality; Eczema
ISSN (print) / ISBN 0160-4120
e-ISSN 1873-6750
Zeitschrift Environment International
Quellenangaben Band: 192, Heft: , Seiten: , Artikelnummer: 109035 Supplement: ,
Verlag Elsevier
Verlagsort The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Research Unit Comparative Microbiome Analysis (COMI)
Research Unit Environmental Simulation (BIOP-EUS)
Förderungen Deutsche Forschungsgemeinschaft
Federal Ministry of Food and Agriculture (BMEL) via FNR (Fachagentur Nachwachsende Rohstoffe)