PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Risha, M.A.* ; Reddy, K.D.* ; Nemani, S.S.P.* ; Jakwerth, C.A. ; Schmidt-Weber, C.B. ; Bahmer, T.* ; Hansen, G.* ; von Mutius, E. ; Rabe, K.F. ; Dittrich, A.M.* ; Grychtol, R.* ; Maison, N. ; Schaub, B.* ; Kopp, M.V.* ; Brinkmann, F.* ; Meiners, S.* ; Jappe, U.* ; Weckmann, M.*

Epigenetic training of human bronchial epithelium cells by repeated rhinovirus infections.

Allergy, DOI: 10.1111/all.16388 (2024)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
BACKGROUND: Humans are subjected to various environmental stressors (bacteria, viruses, pollution) throughout life. As such, an inherent relationship exists between the effect of these exposures with age. The impact of these environmental stressors can manifest through DNA methylation (DNAm). However, whether these epigenetic effects selectively target genes, pathways, and biological regulatory mechanisms remains unclear. Due to the frequency of human rhinovirus (HRV) infections throughout life (particularly in early development), we propose the use of HRV under controlled conditions can model the effect of multiple exposures to environmental stressors. METHODS: We generated a prediction model by combining transcriptome and DNAm datasets from human epithelial cells after repeated HRV infections. We applied a novel experimental statistical design and method to systematically explore the multifaceted experimental space (number of infections, multiplicity of infections and duration). Our model included 35 samples, each characterized by the three parameters defining their infection status. RESULTS: Trainable genes were defined by a consistent linear directionality in DNAm and gene expression changes with successive infections. We identified 77 trainable genes which could be further explored in future studies. The identified methylation sites were tracked within a pediatric cohort to determine the relative changes in candidate-trained sites with disease status and age. CONCLUSIONS: Repeated viral infections induce an immune training response in bronchial epithelial cells. Training-sensitive DNAm sites indicate alternate divergent associations in asthma compared to healthy individuals. Our novel model presents a robust tool for identifying trainable genes, providing a foundation for future studies.
Impact Factor
Scopus SNIP
Altmetric
12.600
0.000
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Airway Epithelium ; Asthma ; Human Rhinovirus ; Trainable Genes ; Trained Immunity
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 0105-4538
e-ISSN 1398-9995
Zeitschrift Allergy
Verlag Wiley
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Institute of Asthma and Allergy Prevention (IAP)
POF Topic(s) 30202 - Environmental Health
Forschungsfeld(er) Allergy
PSP-Element(e) G-505400-001
G-503300-001
Förderungen Federal Ministry of Education and Science: German Center for Lung Research / Deutsches Zentrum für Lungenforschung (DZL) DZL 001C1
DOI 10.1111/all.16388
Scopus ID 85208580694
PubMed ID 39513674
Erfassungsdatum 2024-11-25
[➜Korrektur melden]