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Eberhard, J.* ; Henning, L.* ; Fülle, L.* ; Knöpper, K.* ; Böhringer, J.* ; Graelmann, F.J.* ; Hänschke, L.* ; Kenzler, J.* ; Brosseron, F.* ; Heneka, M.T.* ; Domingos, A.I.* ; Eyerich, S. ; Lochner, M.* ; Weighardt, H.* ; Bedner, P.* ; Steinhauser, C.* ; Förster, I.*

Ablation of CCL17-positive hippocampal neurons induces inflammation-dependent epilepsy.

Epilepsia, DOI: 10.1111/epi.18200 (2024)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
OBJECTIVE: Neuronal cell death and neuroinflammation are characteristic features of epilepsy, but it remains unclear whether neuronal cell death as such is causative for the development of epileptic seizures. To test this hypothesis, we established a novel mouse line permitting inducible ablation of pyramidal neurons by inserting simian diphtheria toxin (DT) receptor (DTR) cDNA into the Ccl17 locus. The chemokine CCL17 is expressed in pyramidal CA1 neurons in adult mice controlling microglial quiescence. METHODS: Seizure activity in CCL17-DTR mice was analyzed by electroencephalographic recordings following treatment with DT for 3 consecutive days. Neuroinflammation and neuronal cell death were evaluated by (immuno)histochemistry. Pharmacological inhibition of TNFR1 signaling was achieved by treatment with XPro1595, a dominant-negative inhibitor of soluble tumor necrosis factor. RESULTS: Neuronal cell death was detectable 7 days (d7) after the first DT injection in heterozygous CCL17-DTR mice. Spontaneous epileptic seizures were observed in the vast majority of mice, often with an initial peak at d6-9, followed by a period of reduced activity and a gradual increase during the 1-month observation period. Microglial reactivity was overt from d5 after DT administration not only in the CA1 region but also in the CA2/CA3 area, shortly followed by astrogliosis. Reactive microgliosis and astrogliosis persisted until d30 and, together with neuronal loss and stratum radiatum shrinkage, reflected important features of human hippocampal sclerosis. Granule cell dispersion was detectable only 3 months after DT treatment. Application of XPro1595 significantly reduced chronic seizure burden without affecting the development of hippocampal sclerosis. SIGNIFICANCE: In conclusion, our data demonstrate that sterile pyramidal neuronal death is sufficient to cause epilepsy in the absence of other pathological processes. The CCL17-DTR mouse line may thus be a valuable model for further mechanistic studies on epilepsy and assessment of antiseizure medication.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Xpro1595 ; Astrogliosis ; Diphtheria Toxin ; Microgliosis ; Neurodegeneration; Tumor-necrosis-factor; Temporal-lobe Epilepsy; Synaptic Reorganization; Dentate Gyrus; Mouse Model; Sclerosis; Seizures; Receptor; Encephalitis
ISSN (print) / ISBN 0013-9580
e-ISSN 1528-1167
Zeitschrift Epilepsia
Verlag Wiley
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Förderungen Deutsches Zentrum für Neurodegenerative Erkrankungen