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Oomen, M.E. ; Rodriguez-Terrones, D. ; Kurome, M.* ; Zakhartchenko, V.* ; Mottes, L. ; Simmet, K.* ; Noll, C. ; Nakatani, T. ; Mourra-Díaz, D.M. ; Aksoy, I.* ; Savatier, P.* ; Goke, J.* ; Wolf, E.* ; Kaessmann, H.* ; Torres-Padilla, M.E.

An atlas of transcription initiation reveals regulatory principles of gene and transposable element expression in early mammalian development.

Cell 188, 1156-1174.e20 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Transcriptional activation of the embryonic genome (EGA) is a major developmental landmark enabling the embryo to become independent from maternal control. The magnitude and control of transcriptional reprogramming during this event across mammals remains poorly understood. Here, we developed Smart-seq+5' for high sensitivity, full-length transcript coverage and simultaneous capture of 5' transcript information from single cells and single embryos. Using Smart-seq+5', we profiled 34 developmental stages in 5 mammalian species and provide an extensive characterization of the transcriptional repertoire of early development before, during, and after EGA. We demonstrate widespread transposable element (TE)-driven transcription across species, including, remarkably, of DNA transposons. We identify 19,657 TE-driven genic transcripts, suggesting extensive TE co-option in early development over evolutionary timescales. TEs display similar expression dynamics across species and species-specific patterns, suggesting shared and divergent regulation. Our work provides a powerful resource for understanding transcriptional regulation of mammalian development.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Tss ; Zga ; Evolutionary Conservation ; Evolutionary Genomics ; Mammalian Preimplantation Development ; Transcriptomics ; Transposable Elements
ISSN (print) / ISBN 0092-8674
e-ISSN 1097-4172
Zeitschrift Cell
Quellenangaben Band: 188, Heft: 4, Seiten: 1156-1174.e20 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Cambridge, Mass.
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Epigenetics and Stem Cells (IES)