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A multiplex polymerase chain reaction assay for the detection of herpes simplex virus, cytomegalovirus, and varicella-zoster virus in cerebrospinal Fluid.
Microorganisms 13:111 (2025)
Viral meningitis poses a significant clinical challenge due to its rapid onset and potential progression to life-threatening encephalitis. Early detection of treatable viral pathogens such as Herpes simplex virus (HSV), Cytomegalovirus (CMV), and Varicella-zoster virus (VZV) is essential for initiating appropriate therapies. However, multiplex PCRs for the rapid and simultaneous detection of these pathogens are scarce due to the complex PCR design and the elaborate validation process using cerebrospinal fluid samples. In this study, we established and validated a novel multiplex PCR assay for detecting HSV, CMV, and VZV in cerebrospinal fluid samples and implemented the assay on a fully automated platform.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Clinical Virology ; Multiplex Pcr ; Viral Meningitis
ISSN (print) / ISBN
2076-2607
e-ISSN
2076-2607
Zeitschrift
Microorganisms
Quellenangaben
Band: 13,
Heft: 1,
Artikelnummer: 111
Verlag
MDPI
Verlagsort
Basel
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Virology (VIRO)