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Kardell, O. ; Gronauer, T.F. ; von Toerne, C. ; Merl-Pham, J. ; König, A.-C. ; Barth, T.K.* ; Mergner, J.* ; Ludwig, C.* ; Tüshaus, J.* ; Giesbertz, P.* ; Breimann, S.* ; Schweizer, L.* ; Müller, T.* ; Kliewer, G.* ; Distler, U.* ; Gomez-Zepeda, D.* ; Popp, O.* ; Qin, D.* ; Teupser, D.* ; Cox, J.* ; Imhof, A.* ; Küster, B.* ; Lichtenthaler, S.F.* ; Krijgsveld, J.* ; Tenzer, S.* ; Mertins, P.* ; Coscia, F.* ; Hauck, S.M.

Multicenter longitudinal quality assessment of MS-based proteomics in plasma and serum.

J. Proteome Res. 24, 1017-1029 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Advancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimization of several bioanalytical parameters such as selectivity, sensitivity, accuracy, and precision. In a joint effort, eight proteomics laboratories belonging to the MSCoreSys initiative including the CLINSPECT-M, MSTARS, DIASyM, and SMART-CARE consortia performed a longitudinal round-robin study to assess the analysis performance of plasma and serum as clinically relevant samples. A variety of LC-MS/MS setups including mass spectrometer models from ThermoFisher and Bruker as well as LC systems from ThermoFisher, Evosep, and Waters Corporation were used in this study. As key performance indicators, sensitivity, precision, and reproducibility were monitored over time. Protein identifications range between 300 and 400 IDs across different state-of-the-art MS instruments, with timsTOF Pro, Orbitrap Exploris 480, and Q Exactive HF-X being among the top performers. Overall, 71 proteins are reproducibly detectable in all setups in both serum and plasma samples, and 22 of these proteins are FDA-approved biomarkers, which are reproducibly quantified (CV < 20% with label-free quantification). In total, the round-robin study highlights a promising baseline for bringing MS-based measurements of serum and plasma samples closer to clinical utility.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Lc-ms/ms ; R Package Mpwr ; Clinical Specimen ; Longitudinal Round-robin Study ; Plasma ; Serum; Biomarker Discovery; Mass; Workflow
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Zeitschrift Journal of Proteome Research
Quellenangaben Band: 24, Heft: 3, Seiten: 1017-1029 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort 1155 16th St, Nw, Washington, Dc 20036 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Förderungen Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
Federal Ministry of Education and Research (BMBF), as part of the National Research Initiatives for Mass Spectrometry in Systems Medicine
German Ministry for Science and Education