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Fleischer, A.B.* ; Amann, B.* ; von Toerne, C. ; Degroote, R.L.* ; Schmalen, A.* ; Weiser, T.* ; Hauck, S.M. ; Deeg, C.A.*

Differential expression of ARG1 and MRC2 in retinal Müller glial cells during autoimmune uveitis.

Biomolecules 15:288 (2025)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Retinal Müller glial cells (RMG) play a crucial role in retinal neuroinflammation, including autoimmune uveitis. Increasing evidence supports their function as active modulators of immune responses and potential atypical antigen-presenting cells (APCs). To further investigate this hypothesis, we conducted a differential proteome analysis of primary equine RMG from healthy controls and horses with equine recurrent uveitis (ERU), a spontaneous model of autoimmune uveitis. This analysis identified 310 proteins with differential abundance. Among these, the Major Histocompatibility Complex (MHC) class II and the enzyme Arginase 1 (ARG1) were significantly enriched in RMG from uveitis-affected horses, whereas Mannose Receptor C-type 2 (MRC2) and its interactor Thrombospondin 1 (THBS1) were more abundant in healthy RMG. The detection of MHC class II in equine RMG, consistent with previous studies, validates the robustness of our approach. Furthermore, the identification of ARG1 and MRC2, together with THBS1, provides new insights into the immunomodulatory and antigen-presenting properties of RMG. Immunohistochemical analyses confirmed the proteomic findings and revealed the spatial distribution of ARG1 and MRC2. ARG1 and MRC2 are thus markers for RMG in the neuroinflammatory or physiological milieu and highlight potential differences in the immune function of RMG, particularly in antigen presentation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Arginase 1 (arg1) ; Retinal Müller Glial Cells (rmg) ; Thrombospondin 1 (thbs1) ; Atypical Antigen Presenting Cell (apc) ; Autoimmune Uveitis ; Equine Recurrent Uveitis (eru) ; Major Histocompatibility Complex Class Ii (mhc Class Ii) ; Mannose Receptor C-type 2 (mrc2) ; Ocular Immune Privilege ; Retinal Neuroinflammation; Epithelium-derived Factor; Equine Recurrent Uveitis; T-cells; Mannose Receptor; Anterior-chamber; Muller Glia; Th17 Cells; Thrombospondin-1; Antigen; Costimulation
ISSN (print) / ISBN 2218-273X
e-ISSN 2218-273X
Zeitschrift Biomolecules
Quellenangaben Band: 15, Heft: 2, Seiten: , Artikelnummer: 288 Supplement: ,
Verlag MDPI
Verlagsort Basel
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Förderungen Deutsche Forschungsgemeinschaft, DFG
Deutsche Forschungsgemeinschaft