PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Waltz, F.* ; Righetto, R.D.* ; Lamm, L. ; Salinas-Giegé, T.* ; Kelley, R.K.* ; Zhang, X.* ; Obr, M.* ; Khavnekar, S.* ; Kotecha, A.* ; Engel, B.D.*

In-cell architecture of the mitochondrial respiratory chain.

Science 387, 1296-1301 (2025)
Postprint DOI PMC
Open Access Green
Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, working in concert to transfer electrons and pump protons. The precise organization of these complexes in native cells is debated. We used in situ cryo-electron tomography to visualize the native structures and organization of several major mitochondrial complexes in Chlamydomonas reinhardtii cells. ATP synthases and respiratory complexes segregate into curved and flat crista membrane domains, respectively. Respiratory complexes I, III, and IV assemble into a respirasome supercomplex, from which we determined a native 5-angstrom (Å) resolution structure showing binding of electron carrier cytochrome c. Combined with single-particle cryo-electron microscopy at 2.4-Å resolution, we model how the respiratory complexes organize inside native mitochondria.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Altmetric
45.800
0.000
2
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cytochrome-c-oxidase; Complex-i; Electron-microscopy; Coupling Mechanism; Atp Synthase; Situ; Supercomplex; Phosphorylation; Visualization; Organization
Sprache englisch
Veröffentlichungsjahr 2025
HGF-Berichtsjahr 2025
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Zeitschrift Science
Quellenangaben Band: 387, Heft: 6740, Seiten: 1296-1301 Artikelnummer: , Supplement: ,
Verlag American Association for the Advancement of Science (AAAS)
Verlagsort 1200 New York Ave, Nw, Washington, Dc 20005 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Artifical Intelligence Cooperation Unit (HAICU)
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-530006-001
Förderungen University of Basel Biozentrum
ERC consolidator grant
SNSF Ambizione grant
Swiss Postdoctoral Fellowship
Swiss National Science Foundation
DOI 10.1126/science.ads8738
WOS ID 001500662800032
PubMed ID 40112058
Erfassungsdatum 2025-05-09
[➜Korrektur melden]