PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Azimzadeh, O. ; Scherthan, H.* ; Yentrapalli, R. ; Barjaktarovic, Z. ; Ueffing, M.* ; Conrad, M. ; Neff, F. ; Calzada-Wack, J. ; Aubele, M. ; Buske, C.* ; Atkinson, M.J. ; Hauck, S.M.* ; Tapio, S.

Label-free protein profiling of formalin-fixed paraffin-embedded (FFPE) heart tissue reveals immediate mitochondrial impairment after ionising radiation.

J. Proteomics 75, 2384-2395 (2012)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Qualitative proteome profiling of formalin-fixed, paraffin-embedded (FFPE) tissue is advancing the field of clinical proteomics. However, quantitative proteome analysis of FFPE tissue is hampered by the lack of an efficient labelling method. The usage of conventional protein labelling on FFPE tissue has turned out to be inefficient. Classical labelling targets lysine residues that are blocked by the formalin treatment. The aim of this study was to establish a quantitative proteomics analysis of FFPE tissue by combining the label-free approach with optimised protein extraction and separation conditions. As a model system we used FFPE heart tissue of control and exposed C57BL/6 mice after total body irradiation using a gamma ray dose of 3 gray. We identified 32 deregulated proteins (p≤0.05) in irradiated hearts 24h after the exposure. The proteomics data were further evaluated and validated by bioinformatics and immunoblotting investigation. In good agreement with our previous results using fresh-frozen tissue, the analysis indicated radiation-induced alterations in three main biological pathways: respiratory chain, lipid metabolism and pyruvate metabolism. The label-free approach enables the quantitative measurement of radiation-induced alterations in FFPE tissue and facilitates retrospective biomarker identification using clinical archives.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
4.878
1.188
31
32
Tags
PROT-CF
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Label-free; Ionising radiation; Proteomics; Mitochondria; FFPE; BOX-BINDING PROTEIN-1; INDUCED GENOMIC INSTABILITY; MASS-SPECTROMETRY; PROTEOMIC ANALYSIS; PRESSURE-OVERLOAD; OXIDATIVE STRESS; EXPRESSION; MEMBRANE; IDENTIFICATION; DYSFUNCTION
Sprache
Veröffentlichungsjahr 2012
HGF-Berichtsjahr 2012
ISSN (print) / ISBN 1874-3919
e-ISSN 1876-7737
Zeitschrift Journal of Proteomics
Quellenangaben Band: 75, Heft: 8, Seiten: 2384-2395 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
Institute of Pathology (PATH)
Institute of Developmental Genetics (IDG)
CF Metabolomics & Proteomics (CF-MPC)
POF Topic(s) 30202 - Environmental Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30204 - Cell Programming and Repair
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Radiation Sciences
Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-500200-001
G-500300-001
G-500500-001
G-505700-001
PubMed ID 22387116
DOI 10.1016/j.jprot.2012.02.019
WOS ID 000303974800010
Scopus ID 84859270363
Erfassungsdatum 2012-04-27
[➜Korrektur melden]