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Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.
STAR Protoc. 6:103774 (2025)
Verlagsversion
DOI
PMC
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Biotechnology And Bioengineering ; Metabolism ; Model Organisms; Pancreatic Beta-cells; C-peptide; Clearance; Model
e-ISSN
2666-1667
Zeitschrift
STAR Protocols
Quellenangaben
Band: 6,
Heft: 2,
Artikelnummer: 103774
Verlag
Elsevier
Verlagsort
Radarweg 29, 1043 Nx Amsterdam, Netherlands
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Experimental Genetics (IEG)
Förderungen
EU Innovative Health Initiative "NHPig"
Deutsche Forschungsgemeinschaft
EU FP7 Network for Initial Training (ITN) "EpiHealthNet"
German Center for Diabetes Research (DZD)
Deutsche Forschungsgemeinschaft
EU FP7 Network for Initial Training (ITN) "EpiHealthNet"
German Center for Diabetes Research (DZD)