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Eckstein, Y.* ; Kessler, B.* ; Hinrichs, A.* ; Novak, I.* ; von Thaden, A.* ; Lorenzen, T.* ; Rathkolb, B. ; Scholz, A.* ; Blutke, A.* ; Koopmans, S.J.* ; Hrabě de Angelis, M. ; Christoffersen, B.* ; Wolf, E.* ; Renner, S.*

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig.

STAR Protoc. 6:103774 (2025)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Biotechnology And Bioengineering ; Metabolism ; Model Organisms
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 6, Heft: 2, Seiten: , Artikelnummer: 103774 Supplement: ,
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)