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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Measurements of molecular size and shape on a chip.
Science 388:eadt5827 (2025)
Size and shape are critical discriminators between molecular species and states. We describe a microchip-based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution conditions. Our method detects differences in molecular weight across at least three orders of magnitude and down to two carbon atoms in small molecules. We quantify the strength of molecular interactions across more than six orders of magnitude in affinity constant and track reactions in real time. Highly parallel measurements on individual molecules serve to characterize sample-state heterogeneity at the highest resolution, offering predictive input to model three-dimensional structure. We further leverage the method's structural sensitivity for diagnostics, exploiting ligand-induced conformational changes in the insulin receptor to sense insulin concentration in serum at the subnanoliter and subzeptomole scale.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Hydrodynamic Properties; Dna; Insulin; Protein; Rna; Binding; Macromolecules; Diffusion; Radii; Mass
ISSN (print) / ISBN
0036-8075
e-ISSN
1095-9203
Zeitschrift
Science
Quellenangaben
Band: 388,
Heft: 6747,
Artikelnummer: eadt5827
Verlag
American Association for the Advancement of Science (AAAS)
Verlagsort
1200 New York Ave, Nw, Washington, Dc 20005 Usa
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Pancreatic Islet Research (IPI)
Förderungen
German Center for Diabetes Research (DZD e.V.)
Federal Ministry of Education and Research (BMBF)
Royal Society of Chemistry Research grant
Royal Society Research Grant
Wellcome LEAP Delta Tissue Program
Biotechnology and Biological Sciences Research Council
European Research Council
Federal Ministry of Education and Research (BMBF)
Royal Society of Chemistry Research grant
Royal Society Research Grant
Wellcome LEAP Delta Tissue Program
Biotechnology and Biological Sciences Research Council
European Research Council