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Tian, C. ; Tiemann, U.* ; Hermann, F.* ; Semb, H.

Protocol for in vitro modeling of specification and morphogenesis of early pancreas development using human pluripotent stem cell-based organoid differentiation.

STAR Protoc. 6:103834 (2025)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Here, we present a protocol to generate key pancreatic cell types in vitro using human pluripotent stem cell (hPSC)-based Matrigel-overlay organoid differentiation. These include multipotent and bipotent progenitors, endocrine progenitors, and hormone-producing endocrine cells. We describe steps for culturing hPSCs as a 2D monolayer, applying a Matrigel overlay to create a 3D epithelial niche, and guiding stepwise differentiation. This system supports live-cell imaging and real-time tracking of morphogenesis and fate decisions, providing a platform for studying organ development and disease. For complete details on the use and execution of this protocol, please refer to Ulf et al.1.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cell Biology ; Cell Differentiation ; Cell Culture ; Developmental Biology ; Organoids ; Stem Cells
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 6, Heft: 2, Seiten: , Artikelnummer: 103834 Supplement: ,
Verlag Elsevier
Verlagsort Radarweg 29, 1043 Nx Amsterdam, Netherlands
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Transl. Stem Cell Research (ITS)
Förderungen
Deutsche Forschungsgemeinschaft (DFG)
Helmholtz Zentrum Munchen
Novo Nordisk Foundation Center for Stem Cell Biology (DanStem) at the University of Copenhagen (NNF)
European Union's Horizon 2020 research and innovation program (ISLET)