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Schuurman, J.L.* ; van Tetering, L.* ; Houthuijs, K.J.* ; Kooijman, P.* ; Gailus-Durner, V. ; Leuchtenberger, S. ; Fuchs, H. ; Hrabě de Angelis, M. ; Engelke, U.F.H.* ; Lefeber, D.J.* ; van Karnebeek, C.D.M.* ; Wevers, R.A.* ; Grgic, A.* ; Balluf, B.* ; Vandenbosch, M.* ; Vreeken, R.* ; Heeren, R.M.A.* ; Berden, G.* ; Oomens, J.* ; Martens, J.W.M.*

Structure elucidation for MALDI mass spectrometry imaging using infrared ion spectroscopy.

Anal. Chem., DOI: 10.1021/acs.analchem.5c00948 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Infrared ion spectroscopy (IRIS) is a tandem mass spectrometry (MS) technique that generates structurally diagnostic vibrational spectra for mass-selected ions trapped in a mass spectrometer. Until now, IRIS applications for biological samples have primarily focused on solution-based analyses, such as body fluids (e.g., plasma and urine) and tissue homogenates, using electrospray ionization (ESI) coupled with liquid chromatography-mass spectrometry (LC-MS). In this study, we have combined matrix-assisted laser desorption/ionization (MALDI) with IRIS for the direct analysis of small molecules from biological tissues on a Fourier-transform ion cyclotron resonance mass spectrometer. We applied this technique alongside MALDI mass spectrometry imaging to analyse brain tissue from two knockout mouse models of l-lysine catabolism disorders: pyridoxine-dependent epilepsy (ALDH7A1) and glutaric aciduria type 1 (GCDH). The MALDI-IRIS platform, now available for users at HFML-FELIX, represents a significant advance in the direct structural characterization of metabolites in complex biological tissues and opens new possibilities for structure elucidation in the field of MALDI mass spectrometry imaging.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Pyridoxine-dependent Epilepsy; Ultraviolet-laser Desorption; Mouse Model; Brain; Ionization; Metabolism; Biomarkers; Antiquitin
ISSN (print) / ISBN 0003-2700
e-ISSN 1520-6882
Zeitschrift Analytical Chemistry
Verlag American Chemical Society (ACS)
Verlagsort 1155 16th St, Nw, Washington, Dc 20036 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)
Förderungen NWO Rekentijd grant
Dutch Research Council (NWO)
Changing Rare Disorders of Lysine Metabolism (CHARLIE) consortium