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Gaber, T.* ; Monecke, T.* ; Grabowski, J.* ; Simon, B.* ; Williams, T.* ; Roman, V. ; Chao, J.* ; Hennig, J.* ; Ephrussi, A.* ; Niessing, D. ; Heber, S.D.*

A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C in the oskar mRNA transport complex.

Cell Rep. 44:115906 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
In the Drosophila female germline, oskar messenger RNA is transported on microtubules from the nurse cells to the posterior pole of the oocyte, where it is translated. Transport of oskar transcripts from the nurse cells into the oocyte requires dynein, while localization of the mRNAs within the oocyte to the posterior pole is dependent upon kinesin-1. Staufen, a double-stranded RNA (dsRNA)-binding protein, has been shown to bind the oskar mRNA transport complex in the oocyte and inactivate dynein; however, it remains unclear how kinesin is activated. Here, using surface plasmon resonance, nuclear magnetic resonance spectroscopy, and RNA imaging within egg chambers, we demonstrate that Staufen directly interacts with Tropomyosin1-I/C (Tm1), a non-canonical kinesin adaptor. This work provides molecular evidence of how Staufen integrates into the oskar messenger ribonucleoprotein (mRNP) complex.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cp: Molecular Biology ; Nmr ; Rna Localization ; Rna-binding Proteins ; Biophysics ; Motor Proteins ; Protein-protein Interactions
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 44, Heft: 7, Seiten: , Artikelnummer: 115906 Supplement: ,
Verlag Cell Press
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)