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Michlmayr, H.* ; Siller, M.* ; Kenjeric, L.* ; Doppler, M.* ; Malachová, A.* ; Hofer, M.J.* ; Hametner, C.* ; Schweiger, W.* ; Steiner, B.* ; Kugler, K.G. ; Mayer, K.F.X. ; Buerstmayr, H.* ; Schuhmacher, R.* ; Krska, R.* ; Labrou, N.E.* ; Papageorgiou, A.C.* ; Adam, G.*

Detoxification of deoxynivalenol by pathogen-inducible tau-class glutathione transferases from wheat.

J. Biol. Chem. 301:110600 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Deoxynivalenol (DON) is a toxicologically relevant trichothecene mycotoxin frequently found in cereal products. It is a virulence factor produced by the plant pathogen Fusarium graminearum during cereal crop infections. Investigating plant defense mechanisms is crucial for understanding plant resistance to F. graminearum and identifying new biocatalysts for DON detoxification. Previous studies identified DON-thiol adducts in cereal samples, indicating partial DON detoxification by glutathione transferases (GSTs). DON possesses two electrophilic centers for thiol conjugation, resulting in either epoxide opening at C13 or Michael addition at C10. At present, information on plant GSTs that catalyze these reactions is limited. In this study, Fusarium-inducible wheat GSTs were identified by analyzing the transcriptome of Fusarium-infected wheat heads. Twelve highly induced genes of the tau and phi GST classes were heterologously expressed and purified, biochemically characterized with model substrates, and assayed for activity with DON. Use of liquid chromatography coupled to mass spectrometry showed that four of the selected tau class GSTs conjugated DON to glutathione (GSH) by epoxide opening (DON-13-GSH) and/or the reversible Michael addition reaction (DON-10-GSH). The crystal structure of a wheat GST (herein designated "TaGST-10") in complex with DON-13-GSH was solved at a resolution of 2.3 Å and provided insights into the binding of DON at the active site of tau class GSTs. Our results corroborate the hypothesis that enzyme-catalyzed, glutathione-mediated DON detoxification may be involved in plant response to Fusarium infection.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Fusarium ; Deoxynivalenol ; Epoxide ; Glutathione Transferase ; Wheat; Fusarium-graminearum; S-transferases; Gene; Proteins; Stress; Trichothecenes; Identification; Expression; Features; Reveals
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Zeitschrift Journal of Biological Chemistry, The
Quellenangaben Band: 301, Heft: 10, Seiten: , Artikelnummer: 110600 Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Verlagsort Radarweg 29, 1043 Nx Amsterdam, Netherlands
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Plant Genome and Systems Biology (PGSB)
Förderungen Austrian Research Promotion Agency FFG
FWF Special Research Program SFB Fusarium
COMET project "Accelerated analysis" of the Austrian Competence Centre for Feed and Food Quality, Safety and Innovation (FFoQSI)
Austrian federal ministry BMK
Austrian federal ministry BMDW
Austrian provinces Lower Austria, Upper Austria and Vienna within the scope of COMET Competence Centers for Excellent Technologies
Erwin-Schroedinger-Fellowship of the Austrian Science Fund (FWF)