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Real-time intraoperative fluorescence imaging system using light-absorption correction.

J. Biomed. Opt. 14:064012 (2009)
DOI PMC
Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras operating in parallel, utilizing a common lens, which allows for the concurrent collection of color, fluorescence, and light attenuation images at the excitation wavelength from the same field of view. The correction is based on a ratio approach of fluorescence over light attenuation images. Color images and video is used for surgical guidance and for registration with the corrected fluorescence images. We showcase the performance metrics of this system on phantoms and animals, and discuss the advantages over conventional epi-illumination systems developed for real-time applications and the limits of validity of corrected epi-illumination fluorescence imaging.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Fluorescence imaging; Epi-illumination; Light attenuation; Intraoperative imaging; Light absorption correction
Sprache englisch
Veröffentlichungsjahr 2009
HGF-Berichtsjahr 2010
ISSN (print) / ISBN 1083-3668
e-ISSN 1560-2281
Quellenangaben Band: 14, Heft: 6, Seiten: , Artikelnummer: 064012 Supplement: ,
Verlag SPIE
Begutachtungsstatus Peer reviewed
POF Topic(s) 30205 - Bioengineering and Digital Health
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505500-001
Scopus ID 77950359684
PubMed ID 20059250
Erfassungsdatum 2009-12-31