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Das, A. ; Delaval, M.N. ; Ihalainen, M.* ; Schnelle-Kreis, J. ; Huber, A. ; Zimmermann, E. ; Di Bucchianico, S. ; Sippula, O.* ; Czech, H. ; Sklorz, M. ; Zimmermann, R.

Technical note: Quantification of ultrafine particle mass deposition in an in vitro air-liquid interface exposure system.

J. Aerosol Sci. 191:106710 (2026)
Verlagsversion DOI
Open Access Hybrid
Creative Commons Lizenzvertrag
Air-Liquid Interface (ALI) cell exposure systems are essential tools for assessing the toxicity of airborne aerosols and engineered nanomaterials in vitro . These systems are increasingly favored for depositing aerosols directly onto cell cultures with improved precision, scalability, and flexibility. However, a significant challenge remains in accurately determining the actual particle deposition, particularly for ultrafine particles (UFP, Dp ≤ 100 nm). This study investigates the chemical-based quantification of UFP mass deposition and the deposition variability across insert positions in an Automated Exposure Station (AES). Multi-well positions in the AES were exposed to soot UFP, rich in polycyclic aromatic hydrocarbons (PAH), and copper UFP for 4 h in independent experiments. To determine the mass deposition of soot UFP, Teflon-coated glass fiber filters were placed at various positions and analyzed to quantify targeted PAH. Similarly, copper UFP was deposited onto empty inserts in different positions, and post-exposure quantification was performed. Mass deposition efficiencies exhibited a high relative variability of 15 % from experiment to experiment, and the position-dependent variability was not significant for either soot UFP or copper UFP. However, compared to the results from a theoretical model, the model significantly underestimated mass deposition by a factor of 5–8. Incorporating an alternative calculation of the boundary layer thickness into the model improved the agreement between model and experimental data. Therefore, for UFP mass deposition results from modeling must be interpreted with care.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter A549 ; Copper ; Dosimetry ; Pah ; Pm0.1 ; Soot; Cell Exposure; Aerosol Deposition; Toxicity
ISSN (print) / ISBN 0021-8502
e-ISSN 1879-1964
Zeitschrift Journal of Aerosol Science
Quellenangaben Band: 191, Heft: , Seiten: , Artikelnummer: 106710 Supplement: ,
Verlag Elsevier
Verlagsort 125 London Wall, London, England
Begutachtungsstatus Peer reviewed
Institut(e) Cooperation Group Comprehensive Molecular Analytics (CMA)
Förderungen TRansportation - Health Assessment of Sources
Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health (HICE)
Helmholtz International Laboratory aeroHEALTH
Bundesministerium fur Wirtschaft und Klimaschutz (BMWK) Zentrales Innovationsprogramm Mittelstand (ZIM)
Bayerischer Projektverbund Ultrafeine Partikel (BAY UFP)
Bavarian State Ministry of the Environment and Consumer Protection