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Sehgal, R.* ; Haacke, N.* ; Maguolo, A.* ; Solari, F.A.* ; Jähnert, M.* ; Gottmann, P.* ; Nilsson, E.* ; Vaag, A.* ; Fischer-Posovszky, P.* ; Werberger, A.* ; Birkenfeld, A.L. ; Fritsche, A. ; Häring, H.-U. ; Sickmann, A.* ; Vogel, H.* ; Ling, C.* ; Ouni, M.* ; Schürmann, A.*

Adipose tissue-derived microRNAs as epigenetic modulators of type 2 diabetes.

BMC Med. 23:678 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
BACKGROUND: White adipose tissue (WAT) dysfunction including an aberrant expression of miRNAs is strongly associated with the risk of developing type 2 diabetes (T2D), with limited evidence linking early changes in the WAT-derived miRNAs and T2D. The present study aims to identify early miRNome changes prognostic for T2D in mice and humans. METHODS: Gonadal (g) WAT of diabetes-resistant and diabetes-prone mice were subjected to multi-omics analyses (transcriptome, miRNome, methylome, proteome). Metabolic phenotypes linked with T2D were correlated with adipose tissue miRNA expression and DNA methylation from 14 monozygotic twin pairs discordant for T2D. Plasma miRNA levels from females at high risk of developing T2D (TÜF study) were included. RESULTS: Adipose tissue of the diabetes-susceptible mice was less insulin sensitive with ~ 200 differentially expressed mature miRNAs compared to diabetes-resistant mice. Integrative analysis of miRNome-transcriptome-proteome identified 227 proteins involved in amino acid metabolism, inflammation, signalling pathways, and insulin resistance. More than 20 differentially expressed miRNAs are located in the imprinted region Dlk1-Gtl2 and Mest (miR-335) potentially regulated by DNA methylation. Imprinted miRNAs also exhibited similar alterations in adipose tissue from monozygotic twin pairs discordant for T2D, with miR-335 expression altered only in females. Moreover, plasma levels of miR-335-5p were negatively correlated with fasting blood glucose in females at high risk of developing T2D. CONCLUSIONS: Early alterations of WAT-derived miRNAs such as miR-335-5p could contribute to systemic metabolic changes associated with the risk of developing T2D.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Adipose Tissue ; Discordant Monozygotic Twins ; Epigenetics ; Imprinting ; Microrna (mirna) ; Multiomics ; New Zealand Obese (nzo) Mice ; Type 2 Diabetes (t2d); Differential Expression; Mir-335-5p; Metabolism; Genes
ISSN (print) / ISBN 1741-7015
e-ISSN 1741-7015
Zeitschrift BMC Medicine
Quellenangaben Band: 23, Heft: 1, Seiten: , Artikelnummer: 678 Supplement: ,
Verlag Bmc
Verlagsort Campus, 4 Crinan St, London N1 9xw, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes Research and Metabolic Diseases (IDM)
Förderungen Stiftelsen fr Strategisk Forskning
Deutsche Diabetes Gesellschaft
Deutsches Institut fr Ernhrungsforschung Potsdam-Rehbrcke (DIfE) (3440)
HORIZON EUROPE Framework Programme
Bundesministerium fr Bildung und Forschung
swedish research council region Skane