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Mayer-Kuckuk, P.

Imaging of Bone Cells In Vivo.

In: Encyclopedia of Bone Biology. 2020. 413-421 (Encyclopedia of Bone Biology)
DOI
Bone cells experience an extraordinarily complex environment. For example, they reside in calcified matrix, are subject to mechanical loading, and their activities are not only dependent on the cross-talk between the osteoblast-osteoclast lineage, but also on a variety of local and systemic factors. Such a complex environment remains difficult to recapitulate in vitro, and consequently the in vivo study of bone cells is important. In this article, imaging techniques for the direct in vivo assessment of cells of the osteoblast and osteoclast lineages are discussed. Imaging techniques are non- or minimally-invasive, thus preserving the bone cell environment. First generation imaging of bone cells established non-invasive molecular imaging of bone cells, using bioluminescence and fluorescence techniques. However, first generation imaging lacked the cellular resolution desirable to address many questions in bone biology. To overcome this hurdle, second generation approaches turned to intra-vital fluorescence imaging, and achieved dynamic recordings of osteoblasts and osteoclasts within the bone space, paving the way for discoveries in bone biology. Lastly, the means developed for imaging bone cells have potential for clinical translation.
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Publikationstyp Artikel: Sammelbandbeitrag/Buchkapitel
ISSN (print) / ISBN [9780128140819, 9780128140826]
Bandtitel Encyclopedia of Bone Biology
Quellenangaben Band: , Heft: , Seiten: 413-421 Artikelnummer: , Supplement: ,
Institut(e) Institute of Experimental Genetics (IEG)