PuSH - Publikationsserver des Helmholtz Zentrums München

Couacault, P. ; Witting, M.

RPLC- and HILIC-based non-targeted metabolomics workflow for blood microsamples.

Metabolomics 22:23 (2026)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
INTRODUCTION: Blood microsampling (BμS) has emerged as an alternative to invasive sampling methods, including blood and plasma sampling. Several studies have shown that BμS are suitable alternatives for analyzing endogenous metabolites and for metabolomics applications. Dried blood spots (DBS) have long been used for clinical applications, particularly for newborn screening. New quantitative BμS have emerged, including volumetric absorptive microsampling (VAMS). OBJECTIVES: We aimed to develop an extraction protocol from BµS for non-targeted metabolomics analysis using a reversed-phase liquid chromatography/mass spectrometry (RPLC-MS) method for the mid- to non-polar metabolome and a hydrophilic interaction chromatography/mass spectrometry (HILIC-MS) method for the polar metabolome, based on existing protocols from the literature. To improve coverage, two new HILIC-MS methods have been developed. METHODS: We used an in-house RPLC-MS method for the analysis of mid- to non-polar metabolites. Two new HILIC-MS/MS methods were developed using 73 chemical reference standards of polar metabolites from various classes. To optimize extraction, five procedures were investigated and compared to identify the most appropriate protocol for extracting metabolites from BµS for non-targeted metabolomics analysis. The final workflow was optimized on both DBS and VAMS. RESULTS AND CONCLUSION: We developed and optimized a 15-minute HILIC-MS method that included column re-equilibration. Our experiments showed that using a 20% H2O/80% MeOH (v/v) mixture for extraction, with sample rehydration, is a good compromise for detecting many metabolite features. Our extraction and LC-MS methodology covered metabolites from many pathways, including amino acids, acylcarnitines, and bile acids.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Blood Microsampling ; Dbs ; Hilic-ms ; Non-targeted Metabolomics ; Rplc-ms ; Vams; Quantitative Bioanalysis
ISSN (print) / ISBN 1573-3882
e-ISSN 1573-3890
Zeitschrift Metabolomics
Quellenangaben Band: 22, Heft: 2, Seiten: , Artikelnummer: 23 Supplement: ,
Verlag Springer
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
Förderungen Helmholtz Zentrum Mnchen - Deutsches Forschungszentrum fr Gesundheit und Umwelt (GmbH) (4209)