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Shelar, D. ; Malankar, G.S.* ; S.Salunkhe, S.* ; R.Singh, P.* ; P.Vaidya, S.* ; D.Chavan, A.* ; V.Pinjari, R.* ; T.Manjare, S.*

Dual role of cyclic Diselenide containing coumarin based scaffold supported with DFT/TD-DFT studies: Superoxide sensor and anti-Cancer agent.

Spectrochim. Acta A 355:127692 (2026)
DOI PMC
Cyclic diselenide-based coumarin compound 4-methyl-7-((naphtho [1,8-cd][1,2]diselenol-3-ylmethylene)amino)-2H-chromen-2-one (probe) was successfully synthesized and characterized using 1H, 13C NMR, HRMS, and FT-IR spectroscopy. The probe endured systematic evaluation against a broad panel of reactive oxygen species (ROS) and biothiols, revealing exceptional selectivity and sensitivity for superoxide relative to other tested analytes. The probe has a lower detection limit of 32 nM for superoxide, as determined through the fluorescence titration method, with a high stock shift of 74 nm. Comparative study revealed that the probe possessed a quantum yield of 1.22%, which increased to 27.3% following reaction with superoxide, indicating a 22-fold increment in fluorescence intensity once oxidised by superoxide. The Density Functional Theory (DFT) studies have predicted that the probe is present in cis conformation with the SeN (imine) bonding. The mechanism of the conversion of the cis-trans isomers of the probe has been investigated, and the interconversion between predicted possible oxidation products is confirmed via combined spectroscopic monitoring (UV-Vis and fluorescence time-course studies), supported by theoretical analysis experimentally. In vitro cell viability assays confirmed that the probe is biocompatible with normal MDCK cells while revealing potent anti-cancer effects against cancerous cells (HeLa and MCF7).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Coumarin ; Scaffold ; Diselenide ; Superoxide ; Cyclic Peptide ; Dual (grammatical Number); Fluorescent-probe; Reactive Oxygen; Luminescent Probes; Selenium; Bodipy; Cells; Recognition; Progress; Anion; Mice
ISSN (print) / ISBN 1386-1425
e-ISSN 1873-3557
Zeitschrift Spectrochimica acta, Part A
Quellenangaben Band: 355, Heft: , Seiten: , Artikelnummer: 127692 Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam [u.a.]
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Förderungen SERB New Delhi, India
University Grant Commission