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Broghammer, H. ; Gebhardt, C. ; Heiker, J.T.

Protocol for measuring mitochondrial respiration in mature human adipocytes using the Seahorse XF analyzer.

STAR Protoc. 7:104520 (2026)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Primary mature human adipocytes are an important tool bridging basic research and clinical medicine by reflecting human biological diversity. Here, we present a protocol for measuring the oxygen consumption rate of mature adipocytes. We describe the steps for isolation of adipocytes from adipose tissue samples, Matrigel embedding, and Seahorse analysis, as well as normalization and data analysis. This protocol enables the investigation of the metabolic function of primary mature human adipocytes in response to drugs or genetic modifications.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cell Biology ; Cell Culture ; Cell Isolation ; Metabolism
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 7, Heft: 2, Seiten: , Artikelnummer: 104520 Supplement: ,
Verlag Elsevier
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Förderungen Deutsche Forschungsgemeinschaft
Studienstiftung des Deutschen Volkes