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Zitzmann, F.D.* ; Remmert, C. ; Rogers, N.K. ; Schmidt, S.* ; Lickert, H. ; Jahnke, H.G.* ; Meier, M.

Real-time, label-free impedimetric monitoring of insulin secretion from three-dimensional stem cell–derived islets.

Biosens. Bioelectron. 306, 118720:118720 (2026)
Verlagsversion DOI PMC
Closed
Creative Commons Lizenzvertrag
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Functional assessment of insulin secretion is essential for the development and application of stem cell–derived β-cell products. Conventional glucose-stimulated insulin secretion (GSIS) assays rely on end-point, batch-averaged enzyme-linked immunosorbent assays (ELISA), limiting temporal resolution and masking functional heterogeneity at the level of individual islet-like clusters. Here, we present a label-free, non-invasive impedance spectroscopy–based approach for real-time monitoring of insulin secretion from individual stem cell–derived islets (SC-islets) cultured on microcavity microelectrode array (MEA) chips. Human induced pluripotent stem cells were differentiated into SC-islets and integrated into microcavity MEAs, enabling three-dimensional confinement and parallel electrical recordings. Impedance measurements during on-chip GSIS revealed characteristic glucose-responsive dynamics that correlated with insulin secretion quantified by ELISA and were suppressed by pharmacological inhibition. Impedance spectroscopy resolved functional responses at the level of individual SC-islets, uncovering cluster-to-cluster variability that was obscured by pooled secretion assays. High-density microcavity MEAs further enabled spatially resolved impedance measurements, revealing intra-cluster heterogeneity of insulin secretion. While impedance spectroscopy provides an indirect readout and glucose stimulation was applied as stepwise changes, this platform enables real-time, scalable, and cluster-resolved functional assessment of three-dimensional islet tissues. The approach establishes a foundation for functional screening of SC-islets, differentiation optimization, drug testing, and future potency assessment workflows.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Electrochemical Impedance Spectroscopy ; Glucose Stimulated Insulin Secretion Gsis ; Hipsc Derived Islets And β-cells ; Label-free Secretion Profiling ; Microcavity Arrays ; Spatial Resolved Monitoring
ISSN (print) / ISBN 0956-5663
e-ISSN 1873-4235
Zeitschrift Biosensors and Bioelectronics
Quellenangaben Band: 306, Heft: , Seiten: 118720 Artikelnummer: 118720 Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
Institute of Diabetes and Regeneration Research (IDR)