This second workshop on noninvasive glucose monitoring was held at the Massachusetts Institute of Technology (MIT) on November 5, 2025 (https://sites.mit.edu/nigm-workshop). Eleven invited speakers, representing industry, academia, clinical practice, and regulatory affairs, gave presentations that covered (1) an overview of the noninvasive glucose monitoring technologies; (2) the state of the art in noninvasive glucose monitoring technologies, such as Near-Infrared (NIR), photoacoustic, photothermal, and Raman spectroscopies; (3) a clinician’s perspective on the impact of the current continuous glucose monitoring devices for patient care; and (4) regulatory considerations. Four posters were also presented by junior researchers in the field.