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Poitout, A.* ; Ugalde, J.M.* ; Gorgues, L.* ; Dongois, A.* ; Scholz, P.* ; Nacry, P.* ; Alcon, C.* ; Fiche, J.* ; Dumont, X.* ; Nollman, M.* ; Jhala, K. ; Schäffner, A. ; Jaillais, Y.* ; Verdoucq, L.* ; Meyer, A.J.* ; Martinière, A.*

Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress.

Proc. Natl. Acad. Sci. U.S.A. 123:e2529740123 (2026)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
The spatiotemporal organization of proteins and lipids within membranes is crucial for ensuring proper cellular signaling. While the segregation of proteins and lipids into membrane nanodomains is well established, it remains unclear whether nanodomains can generate gradients of small diffusible molecules. In plants, reactive oxygen species (ROS), especially hydrogen peroxide (H2O2), act as key signaling molecules in response to environmental stimuli such as osmotic stress. However, how extracellular H2O2 affects intracellular signaling has remained unknown. Here, we show that osmotic stimulation induces the formation of localized, H2O2-rich nanoenvironments at the cytoplasmic face of the plasma membrane (PM) in Arabidopsis root cells. Using a PM-tethered H2O2 biosensor, we found that these oxidized nanodomains arise from the clustering of RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs) and RHO OF PLANTS 6 (ROP6), in coordination with aquaporin-mediated H2O2 transport via the PLASMA MEMBRANE INTRINSIC PROTEIN2;7 (PIP2;7). These local redox hotspots at the PM create a feedforward loop in which H2O2 enhances ROP6 nanoclustering, thereby amplifying ROS signaling. Disruption of H2O2 production or transport dampens both ROP6 clustering and anisotropic cell expansion, indicating a crucial role for spatially confined redox signaling in regulating plant growth under osmotic stress. Our findings propose a model in which ROP6/RBOHD-F/PIP2;7 nanodomains function as discrete redox signaling units, redefining ROS signaling at the PM as a structured, signal-specific, and compartmentalized process.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Reactive Oxygen Species ; Cell Signaling ; Osmotic Shock ; Membrane ; Arabidopsis ; Nadph Oxidase ; Extracellular ; Intracellular; Nadph Oxidase; Thiol Peroxidase; Receptor Kinase; Arabidopsis; Gtpase; Binding; H2o2; Accumulation; Immunity; Protein
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Zeitschrift Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Band: 123, Heft: 26, Seiten: , Artikelnummer: e2529740123 Supplement: ,
Verlag National Academy of Sciences
Verlagsort 2101 Constitution Ave Nw, Washington, Dc 20418 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Environmental Simulation (EUS)
Institute of Biochemical Plant Pathology (BIOP)
Förderungen DAAD, Germany
European Molecular Biology Organization (EMBO)
EC | European Research Council (ERC)
Agence Nationale de la Recherche (ANR)
Marie Curie Sklodowska Action
Deutsche Forschungsgemeinschaft (DFG)