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Georgi, J.A.* ; Hartwig, M.* ; Friedrich, U.A. ; Krause, M.* ; Dahl, A.* ; Altmann, H.* ; Stauch, T.* ; Middeke, J.M.* ; Bornhäuser, M.* ; Bugert, P.* ; Thiede, C.* ; Trautmann-Grill, K.*

RNA sequencing indicates distinct platelet transcriptomic changes in immune thrombocytopenia.

J. Thromb. Haemost. 24, 3723-3731 (2026)
Verlagsversion Forschungsdaten DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
BACKGROUND: In immune thrombocytopenia (ITP), platelet-intrinsic alterations and their potential contribution to disease pathogenesis remain incompletely understood. Since platelets, although anucleate, retain a functional transcriptome, transcriptomic profiling may provide insight into disease-associated changes. OBJECTIVES: To investigate platelet-intrinsic transcriptomic changes in ITP and to determine whether these alterations are disease-specific compared with non-immune thrombocytopenia. METHODS: Total RNA sequencing was performed on purified platelets from patients with active ITP (n=6), chemotherapy-induced thrombocytopenia as non-immune thrombocytopenic control (n=6), ITP in treatment-free remission (n=6), and healthy controls (n=8). RESULTS: Platelets from patients with active ITP exhibited higher total RNA content, consistent with enrichment of young, recently released platelets. Transcriptomic analysis identified a profile dominated by platelet activation pathways, including integrin and calcium-dependent signaling, cytoskeletal remodeling, and vesicle trafficking. In contrast, genes involved in mitochondrial biogenesis and oxidative phosphorylation were downregulated, accompanied by a reduced proportion of mitochondrial-derived RNA. Although both active ITP and chemotherapy-induced thrombocytopenia showed elevated RNA content relative to healthy controls, reflecting enhanced platelet turnover, the coordinated upregulation of activation-associated transcripts was unique to active ITP. Platelets from ITP patients in treatment-free remission showed partial normalization of the transcriptional profile, with loss of the activation signature and intermediate expression patterns between active ITP and healthy controls. CONCLUSION: RNA sequencing data show distinct platelet transcriptomic changes in ITP marked by coordinated upregulation of platelet activation pathways and reduced mitochondrial gene expression. This profile is consistent with an activation-primed, metabolically altered platelet state and may reflect disease-related changes in platelet biology in ITP.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Immune Thrombocytopenia ; Rna Sequencing ; Platelet Activation ; Platelet Transcriptome; Players
ISSN (print) / ISBN 1538-7933
e-ISSN 1538-7836
Zeitschrift Journal of Thrombosis and Haemostasis
Quellenangaben Band: 24, Heft: 10, Seiten: 3723-3731 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort 101 Park Avenue, 24th Floor, New York, Ny, United States
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
Förderungen MeDDrive program of the Faculty of Medicine at Dresden University of Technology