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Georgi, J.A.* ; Hartwig, M.* ; Friedrich, U.A. ; Krause, M.* ; Dahl, A.* ; Altmann, H.* ; Stauch, T.* ; Middeke, J.M.* ; Bornhäuser, M.* ; Bugert, P.* ; Thiede, C.* ; Trautmann-Grill, K.*

RNA sequencing indicates distinct platelet transcriptomic changes in immune thrombocytopenia.

J. Thromb. Haemost., DOI: 10.1016/j.jtha.2026.06.034 (2026)
Postprint DOI PMC
Open Access Green
BACKGROUND: In immune thrombocytopenia (ITP), platelet-intrinsic alterations and their potential contribution to disease pathogenesis remain incompletely understood. Since platelets, although anucleate, retain a functional transcriptome, transcriptomic profiling may provide insight into disease-associated changes. OBJECTIVES: To investigate platelet-intrinsic transcriptomic changes in ITP and to determine whether these alterations are disease-specific compared with non-immune thrombocytopenia. METHODS: Total RNA sequencing was performed on purified platelets from patients with active ITP (n=6), chemotherapy-induced thrombocytopenia as non-immune thrombocytopenic control (n=6), ITP in treatment-free remission (n=6), and healthy controls (n=8). RESULTS: Platelets from patients with active ITP exhibited higher total RNA content, consistent with enrichment of young, recently released platelets. Transcriptomic analysis identified a profile dominated by platelet activation pathways, including integrin and calcium-dependent signaling, cytoskeletal remodeling, and vesicle trafficking. In contrast, genes involved in mitochondrial biogenesis and oxidative phosphorylation were downregulated, accompanied by a reduced proportion of mitochondrial-derived RNA. Although both active ITP and chemotherapy-induced thrombocytopenia showed elevated RNA content relative to healthy controls, reflecting enhanced platelet turnover, the coordinated upregulation of activation-associated transcripts was unique to active ITP. Platelets from ITP patients in treatment-free remission showed partial normalization of the transcriptional profile, with loss of the activation signature and intermediate expression patterns between active ITP and healthy controls. CONCLUSION: RNA sequencing data show distinct platelet transcriptomic changes in ITP marked by coordinated upregulation of platelet activation pathways and reduced mitochondrial gene expression. This profile is consistent with an activation-primed, metabolically altered platelet state and may reflect disease-related changes in platelet biology in ITP.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Immune Thrombocytopenia ; Rna Sequencing ; Platelet Activation ; Platelet Transcriptome
ISSN (print) / ISBN 1538-7933
e-ISSN 1538-7836
Zeitschrift Journal of Thrombosis and Haemostasis
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)