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Rosenberger, C. ; Heller, C. ; Basiewicz, T.M.* ; Lehmann, S. ; Sarangova, V.* ; Abdelgawad, H. ; Schubert, U. ; Schmid, J. ; Werner, C.* ; Bornstein, S.R. ; Welzel, P.B.* ; Ludwig, B.

High-density microwell arrays enable controlled pseudoislet engineering for diabetes cell therapy.

Biomed. Mater. 21:045023 (2026)
Verlagsversion DOI
Open Access Hybrid
Creative Commons Lizenzvertrag
Macroencapsulation systems for pancreatic islet delivery are an emerging strategy for cell replacement therapy in type 1 diabetes, aimed at eliminating systemic immunosuppression and enabling the use of alternative cell sources. A critical design challenge is to achieve high packing densities while maintaining sufficient oxygen and nutrient supply to ensure islet viability and function. This design-focused study presents a one-step method for the simultaneous and defined formation and spatial arrangement of uniformly sized pancreatic pseudoislets using high-density concave microwell arrays with potential for future integration into macroencapsulation devices. Microwells with diameters between 130 and 200 µm were arranged in a hexagonal pattern with inter-well spacings of 50 or 100 µm to maximize the packing density while maintaining separation between clusters. The effects of microwell depth, diameter, and inter-well spacing on pseudoislet size, morphology, viability, and glucose-stimulated insulin secretion were systematically evaluated using reaggregated rat islet cells as a standardized and well-characterized model system under normoxic and hypoxic conditions. The optimized microwell configuration enabled efficient pseudoislet formation with a tailorable, uniform size, high viability, and preserved insulin secretory function, even at high densities. This scalable microwell array technology addresses key limitations of current pseudoislet culture and transplantation methods, thus providing a base for improved macroencapsulation device design in diabetes cell therapy.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Concave Microwell ; Diabetes ; High-density Microwell Array ; Islet Clustering ; Macroencapsulation ; Microarrangement ; Pseudoislets; Pancreatic-islets; Aggregate Formation; Device; Culture; Transplantation; Spheroids; Efficient; Polymers; Size
ISSN (print) / ISBN 1748-6041
e-ISSN 1748-605X
Zeitschrift Biomedical Materials
Quellenangaben Band: 21, Heft: 4, Seiten: , Artikelnummer: 045023 Supplement: ,
Verlag Institute of Physics Publishing (IOP)
Verlagsort No.2 The Distillery, Glassfields, Avon Street, Bristol, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
Förderungen Deutsche Forschungsgemeinschaft